About: Cell lines from the soft tick Ornithodoros moubata

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Primary cell cultures (n = 16) were initiated from tissues of embryonic and neonatal larval O. moubata following methods developed for hard ticks. After maintenance for 20-25 months in vitro, cell multiplication commenced in surviving cultures, leading to the establishment of 6 cell lines designated OME/CTVM21, 22, 24, 25, 26 and 27. All lines are maintained at 28 degrees C, with subculture at 2-8 week intervals. The cultures comprise heterogeneous populations of large cells of 15-100 microm in diameter, often with finger-like protrusions and/or intracellular crystals, rarely attached, predominantly floating and forming clumps or hollow multicellular vesicles up to 1 mm in diameter. Attempts to cryopreserve the cells are described. Tick-borne encephalitis virus has been serially passaged 10 times in OME/CTVM21 cells without significant decrease in virus production and with no change in its biological properties as shown by the size and morphology of plaques produced. Primary cell cultures (n = 16) were initiated from tissues of embryonic and neonatal larval O. moubata following methods developed for hard ticks. After maintenance for 20-25 months in vitro, cell multiplication commenced in surviving cultures, leading to the establishment of 6 cell lines designated OME/CTVM21, 22, 24, 25, 26 and 27. All lines are maintained at 28 degrees C, with subculture at 2-8 week intervals. The cultures comprise heterogeneous populations of large cells of 15-100 microm in diameter, often with finger-like protrusions and/or intracellular crystals, rarely attached, predominantly floating and forming clumps or hollow multicellular vesicles up to 1 mm in diameter. Attempts to cryopreserve the cells are described. Tick-borne encephalitis virus has been serially passaged 10 times in OME/CTVM21 cells without significant decrease in virus production and with no change in its biological properties as shown by the size and morphology of plaques produced. (en)
Title	Cell lines from the soft tick Ornithodoros moubata Cell lines from the soft tick Ornithodoros moubata (en)
skos:prefLabel	Cell lines from the soft tick Ornithodoros moubata Cell lines from the soft tick Ornithodoros moubata (en)
skos:notation	RIV/60077344:_____/09:00335220!RIV10-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA524/08/1509), P(LC06009), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Růžek, Daniel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	306223
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00335220
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ornithodoros moubata; soft tick; tick-borne encephalitis (en)
http://linked.open.../riv/klicoveSlovo	tick-borne encephalitis Ornithodoros moubata soft tick
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[AABFE0A16DC3]
http://linked.open...i/riv/nazevZdroje	Experimental and Applied Acarology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for molecular ecology of vectors and pathogens Molecular pathogenesis of tick-borne encephalitis
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Růžek, Daniel Bell-Sakyi, L. Gould, E. A.
http://linked.open...ain/vavai/riv/wos	000270430900005
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0168-8162
number of pages	11 (xsd:int)

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