About: Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria

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About: Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Kinetoplastids encode a single nuclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplasmic protein synthesis but cannot decode the mitochondrial UGA codons. Following mitochondrial import, this problem is circumvented in Trypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant mitochondrial UGA tryptophan codons. This tRNA also undergoes an unusual thiolation at position 33. In other organisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1. We show that the mitochondria-targeted TbNfs, is essential for thiolation of both cytosolic and mitochondrial tRNAs. Furthermore, thiolation affects tRNA stability in the cytoplasm but more surprisingly acts as a negative determinant for the essential C to U editing in T. brucei. This provides a first line of evidence for mitochondrial C to U editing regulation in this system. Kinetoplastids encode a single nuclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplasmic protein synthesis but cannot decode the mitochondrial UGA codons. Following mitochondrial import, this problem is circumvented in Trypanosoma brucei by specifically editing the tRNA(Trp) anticodon to UCA, which can now decode the predominant mitochondrial UGA tryptophan codons. This tRNA also undergoes an unusual thiolation at position 33. In other organisms, tRNA thiolation is mediated by the cysteine desulfurase, Nfs1. We show that the mitochondria-targeted TbNfs, is essential for thiolation of both cytosolic and mitochondrial tRNAs. Furthermore, thiolation affects tRNA stability in the cytoplasm but more surprisingly acts as a negative determinant for the essential C to U editing in T. brucei. This provides a first line of evidence for mitochondrial C to U editing regulation in this system. (en)
Title	Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria (en)
skos:prefLabel	Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria (en)
skos:notation	RIV/60077344:_____/09:00334949!RIV10-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06129), P(GA204/06/1558), P(LC07032), Z(AV0Z60220518)
http://linked.open...iv/cisloPeriodika	36
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Poliak, Pavel Lukeš, Julius Paris, Zdeněk Long, Shaojun
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	346273
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00334949
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	T. brucei; tRNA; 2-thiolation; Fe-S cluster; editing (en)
http://linked.open.../riv/klicoveSlovo	tRNA 2-thiolation Fe-S cluster T. brucei editing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C7099E7606F7]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Model organisms as a key to the understanding of functions of disease-related human genes Center for Functional Genetics Functional analysis of mitochondrial proteins in Trypanosoma brucei
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	284
http://linked.open...iv/tvurceVysledku	Alfonzo, J. D. Lukeš, Julius Paris, Zdeněk Long, Shaojun Poliak, Pavel RUBIO, M. A. T. Wohlgamuth-Benedum, J. M.
http://linked.open...ain/vavai/riv/wos	000269380200008
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni
issn	0021-9258
number of pages	7 (xsd:int)

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