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rdf:type
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Description
| - All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation of the Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import.
- All mitochondrial tRNA genes are encoded in nucleus of T. brucei and thus all tRNAs have to be imported from the cytosol. We have investigated that silencing of the cysteine desulfurase, TbNfs, the key enzyme in tRNA thiolation (s2U) and Fe-S cluster formation in vivo, has no effect on tRNA partitioning. This is especially surprising in light of a recent report suggesting that in L. tropica the Rieske Fe-S protein is an essential component of the RNA import complex. We also show that down-regulation of the Rieske protein by RNA interference, similar to the TbNfs knockdowns, has no effect on import. The data presented here supports: s(2)U is not a negative determinant for tRNA import; the Rieske protein is not an essential component of the import machinery, and since the Rieske protein is essential for respiration and maintenance of inner mitochondrial membrane potential, neither process plays a critical role in tRNA import. (en)
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Title
| - Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein
- Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein (en)
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skos:prefLabel
| - Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein
- Mitochondrial tRNA import in Trypanosoma brucei is independent of thiolation and the Rieske protein (en)
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skos:notation
| - RIV/60077344:_____/09:00334944!RIV10-MSM-60077344
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(2B06129), P(GA204/06/1558), P(LC07032), Z(AV0Z60220518)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/09:00334944
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - T. brucei; tRNA import; 2-thiolation; RIC; Rieske; Fe-S cluster (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Alfonzo, J. D.
- Lukeš, Julius
- Paris, Zdeněk
- RUBIO, M. A. T.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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