About: Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio     Goto   Sponge   NotDistinct   Permalink

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  • We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the nematode’ (TTAGGC)n, arthropod’ (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance.
  • We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the nematode’ (TTAGGC)n, arthropod’ (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance. (en)
Title
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio (en)
skos:prefLabel
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio
  • Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio (en)
skos:notation
  • RIV/60077344:_____/09:00331350!RIV10-AV0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/06/1860), P(KJB501410901), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 345683
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/09:00331350
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Acanthocephala; Platyhelminthes; chromosomes (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CA - Kanada
http://linked.open...ontrolniKodProRIV
  • [16B27EEC5BB8]
http://linked.open...i/riv/nazevZdroje
  • Genome
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 52
http://linked.open...iv/tvurceVysledku
  • Špakulová, M.
  • Vítková, Magda
  • Koubková, B.
  • Bombarová, M.
http://linked.open...ain/vavai/riv/wos
  • 000272206700001
http://linked.open...n/vavai/riv/zamer
issn
  • 0831-2796
number of pages
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