About: Cloning, expression and characterisation of a promising mosquitocidal gene

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new mosquitocidal gene, cyt1Aa6, was isolated and cloned from the novel Bacillus thuringiensis strain LLP29, previously isolated from the phylloplane of Magnolia denudata. Nucleotide sequence analysis of cyt1Aa6 indicated that the open reading frame consisted of 750 base pairs, encoding 249 amino acid sequences with a calculated molecular weight of 27.3681 kDa and a PI value of 4.77. An homological comparison revealed that the cyt1Aa6 amino acid sequence was 99% identical with those of known Cyt1Aa proteins. In addition, the cyt1Aa6 gene was successfully expressed in Escherichia coli BL21. Bioassays on Aedes albopictus showed that Bt LLP29 and the expressed BL21 were both toxic to 3rd-instar mosquito larvae. The isolation of cyt1Aa6 provides new opportunities for selecting new strains and to obtain novel B. thuringiensis products based on its toxins. A new mosquitocidal gene, cyt1Aa6, was isolated and cloned from the novel Bacillus thuringiensis strain LLP29, previously isolated from the phylloplane of Magnolia denudata. Nucleotide sequence analysis of cyt1Aa6 indicated that the open reading frame consisted of 750 base pairs, encoding 249 amino acid sequences with a calculated molecular weight of 27.3681 kDa and a PI value of 4.77. An homological comparison revealed that the cyt1Aa6 amino acid sequence was 99% identical with those of known Cyt1Aa proteins. In addition, the cyt1Aa6 gene was successfully expressed in Escherichia coli BL21. Bioassays on Aedes albopictus showed that Bt LLP29 and the expressed BL21 were both toxic to 3rd-instar mosquito larvae. The isolation of cyt1Aa6 provides new opportunities for selecting new strains and to obtain novel B. thuringiensis products based on its toxins. (en)
Title	Cloning, expression and characterisation of a promising mosquitocidal gene Cloning, expression and characterisation of a promising mosquitocidal gene (en)
skos:prefLabel	Cloning, expression and characterisation of a promising mosquitocidal gene Cloning, expression and characterisation of a promising mosquitocidal gene (en)
skos:notation	RIV/60077344:_____/09:00330288!RIV10-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B08003), Z(AV0Z50070508)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Gelbič, Ivan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	307309
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00330288
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bacillus thuringiensis; cyt1Aa6; expression (en)
http://linked.open.../riv/klicoveSlovo	Bacillus thuringiensis expression cyt1Aa6
http://linked.open...odStatuVydavatele	IN - Indická republika
http://linked.open...ontrolniKodProRIV	[33F1AFA8EC0D]
http://linked.open...i/riv/nazevZdroje	INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Changes of mosquito biodiversity - vectores of pathogenic agents in relationship with weather changeability
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Gelbič, Ivan Zhang, L. Guan, X. Huang, E. Guan, Y. Guan, Ch.
http://linked.open...ain/vavai/riv/wos	000270615700003
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems
issn	0019-5189
number of pages	5 (xsd:int)

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