About: Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During early summer and autumn 2005, surveys were conducted in apple and pear production plantations and cultivar collection in the Research and Breeding Institute of Pomology, Holovousy (East Bohemia). A total of 14 pear and 14 apple trees having symptoms of branch proliferation, dwarf, premature leaf reddening and also asymptomatic trees were tested for the presence of phytoplasmas. Nested PCR procedures were employed with different sets of primers to amplify phytoplasma 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Among the plants tested 7 pear and 7 apple trees were positive in nested PCR. Phytoplasmas belonging to apple proliferation group, subgroup 16Sr X-C and 16Sr X-A were identified in 6 pear and 3 apple trees, respectively. RFLP analyses of phytoplasma 16Sr DNAs (R16F2/R16R2 nested PCR products) from 2 apple and I pear trees after digestion with endonuclease MseI is very similar to profile of phytoplasmas belonging to 16SrI-C subgroup. During early summer and autumn 2005, surveys were conducted in apple and pear production plantations and cultivar collection in the Research and Breeding Institute of Pomology, Holovousy (East Bohemia). A total of 14 pear and 14 apple trees having symptoms of branch proliferation, dwarf, premature leaf reddening and also asymptomatic trees were tested for the presence of phytoplasmas. Nested PCR procedures were employed with different sets of primers to amplify phytoplasma 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Among the plants tested 7 pear and 7 apple trees were positive in nested PCR. Phytoplasmas belonging to apple proliferation group, subgroup 16Sr X-C and 16Sr X-A were identified in 6 pear and 3 apple trees, respectively. RFLP analyses of phytoplasma 16Sr DNAs (R16F2/R16R2 nested PCR products) from 2 apple and I pear trees after digestion with endonuclease MseI is very similar to profile of phytoplasmas belonging to 16SrI-C subgroup. (en)
Title	Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic (en)
skos:prefLabel	Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic (en)
skos:notation	RIV/60077344:_____/08:00339355!RIV10-AV0-60077344
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(1QS500510558), V, Z(AV0Z50510513)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Fránová, Jana Petrzik, Karel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	380335
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/08:00339355
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	apples; pears; phytoplasmas; PCR (en)
http://linked.open.../riv/klicoveSlovo	PCR phytoplasmas pears apples
http://linked.open...ontrolniKodProRIV	[69031522F4A6]
http://linked.open...v/mistoKonaniAkce	Antalya
http://linked.open...i/riv/mistoVydani	Leueven 1
http://linked.open...i/riv/nazevZdroje	Proceedings of the twentieth international symposium on virus and virus-liked diseases of temperature fruit crops-fruit tree diseases
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of phytopathogenes occurrence and its genetic variants
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Petrzik, Karel Fránová, Jana Paprštein, F. Kučerová, J.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000256218900051
http://linked.open.../riv/zahajeniAkce	2006-05-22 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
number of pages	10 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS Acta Horticulturea
https://schema.org/isbn	978-90-6605-080-8

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