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rdf:type
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Description
| - During early summer and autumn 2005, surveys were conducted in apple and pear production plantations and cultivar collection in the Research and Breeding Institute of Pomology, Holovousy (East Bohemia). A total of 14 pear and 14 apple trees having symptoms of branch proliferation, dwarf, premature leaf reddening and also asymptomatic trees were tested for the presence of phytoplasmas. Nested PCR procedures were employed with different sets of primers to amplify phytoplasma 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Among the plants tested 7 pear and 7 apple trees were positive in nested PCR. Phytoplasmas belonging to apple proliferation group, subgroup 16Sr X-C and 16Sr X-A were identified in 6 pear and 3 apple trees, respectively. RFLP analyses of phytoplasma 16Sr DNAs (R16F2/R16R2 nested PCR products) from 2 apple and I pear trees after digestion with endonuclease MseI is very similar to profile of phytoplasmas belonging to 16SrI-C subgroup.
- During early summer and autumn 2005, surveys were conducted in apple and pear production plantations and cultivar collection in the Research and Breeding Institute of Pomology, Holovousy (East Bohemia). A total of 14 pear and 14 apple trees having symptoms of branch proliferation, dwarf, premature leaf reddening and also asymptomatic trees were tested for the presence of phytoplasmas. Nested PCR procedures were employed with different sets of primers to amplify phytoplasma 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Among the plants tested 7 pear and 7 apple trees were positive in nested PCR. Phytoplasmas belonging to apple proliferation group, subgroup 16Sr X-C and 16Sr X-A were identified in 6 pear and 3 apple trees, respectively. RFLP analyses of phytoplasma 16Sr DNAs (R16F2/R16R2 nested PCR products) from 2 apple and I pear trees after digestion with endonuclease MseI is very similar to profile of phytoplasmas belonging to 16SrI-C subgroup. (en)
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Title
| - Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic
- Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic (en)
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skos:prefLabel
| - Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic
- Molecular identification of phytoplasmas in cultivar collection and production plantations of apple and pear trees in the Czech Republic (en)
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skos:notation
| - RIV/60077344:_____/08:00339355!RIV10-AV0-60077344
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1QS500510558), V, Z(AV0Z50510513)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00339355
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - apples; pears; phytoplasmas; PCR (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - Proceedings of the twentieth international symposium on virus and virus-liked diseases of temperature fruit crops-fruit tree diseases
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Petrzik, Karel
- Fránová, Jana
- Paprštein, F.
- Kučerová, J.
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http://linked.open...vavai/riv/typAkce
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http://linked.open...ain/vavai/riv/wos
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
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https://schema.org/isbn
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