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| Description
| - Provedli jsme srovnávací studii vnímavosti různých klíštěcích buněčných linií k infekci virem klíšťové encefalitidy, prototypovým kmenem Neudoerfl. Množení viru bylo vyšetřováno v buňkách odvozených od vektorového klíštěte Ixodes ricinus L. (IRE IRE/CTVM18, 19 a 20), jakož i nevektorových klíšťat I. scapularis Say (IDE2), Boophilus microplus Canestrini (BME/CTVM2), Hyalomma anatolicum anatolicum Koch (HAE/CTVM9), Rhipicephalus appendiculatus Neumann (RA-257) a nyní vytvořené linie odvozené z klíštěte Ornithodoros moubata Murray (OME/CTVM21 a 22). Všechny testované linie byly k infekci virem KE citlivé a vykazovaly produktivní infekci bez cytopatického efektu. Nicméně, byl pozorován rozdíl v titru viru mezi vektorovými a nevektorovými klíšťaty, kdy buňky I. ricinus produkovaly 100-1000x více viru oproti nevektorovým liniím. Nejnižší virová produkce byla pozorována u O. moubata a R. appendiculatus. (cs)
- We undertook a comparative study of the susceptibility of different tick cell lines to infection with the European subtype of tick-borne encephalitis virus (TBEV), prototype strain Neudoerfl. The growth of TBEV was studied in lines derived from vector Ixodes ricinus ticks (IRE/CTVM18, 19, and 20), as well as nonvector ticks, I. scapularis (IDE2), Boophilus microplus (BME/CTVM2), Hyalomma anatolicum (HAE/CTVM9), Rhipicephalus appendiculatus (RA-257) and Ornithodoros moubata (OME/CTVM21 and 22). All the tick cell lines tested were susceptible to infection by TBEV and the virus caused productive infection without any cytopathic effect. However, there was a clear difference between the TBEV growth in vector and non-vector cell lines, since I. ricinus cell lines produced 100–1000-fold higher virus yield than the non-vector cell lines. The lowest virus production was observed in O. moubata and R. appendiculatus cell lines.
- We undertook a comparative study of the susceptibility of different tick cell lines to infection with the European subtype of tick-borne encephalitis virus (TBEV), prototype strain Neudoerfl. The growth of TBEV was studied in lines derived from vector Ixodes ricinus ticks (IRE/CTVM18, 19, and 20), as well as nonvector ticks, I. scapularis (IDE2), Boophilus microplus (BME/CTVM2), Hyalomma anatolicum (HAE/CTVM9), Rhipicephalus appendiculatus (RA-257) and Ornithodoros moubata (OME/CTVM21 and 22). All the tick cell lines tested were susceptible to infection by TBEV and the virus caused productive infection without any cytopathic effect. However, there was a clear difference between the TBEV growth in vector and non-vector cell lines, since I. ricinus cell lines produced 100–1000-fold higher virus yield than the non-vector cell lines. The lowest virus production was observed in O. moubata and R. appendiculatus cell lines. (en)
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| Title
| - Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks
- Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks (en)
- Replikace viru klíšťové encefalitidy (Evropský subtyp) v buněčných liniích odvozených od vektorově kompetentních a nekompetentních klíšťat (cs)
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| skos:prefLabel
| - Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks
- Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks (en)
- Replikace viru klíšťové encefalitidy (Evropský subtyp) v buněčných liniích odvozených od vektorově kompetentních a nekompetentních klíšťat (cs)
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| skos:notation
| - RIV/60077344:_____/08:00318392!RIV09-GA0-60077344
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/1479), P(GA524/08/1509), P(LC06009), Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00318392
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - tick-borne encephalitis virus; ticks; vector competence (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Grubhoffer, Libor
- Kopecký, Jan
- Růžek, Daniel
- Bell-Sakyi, L.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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