About: Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea     Goto   Sponge   NotDistinct   Permalink

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Description
  • Fluorogenní substrát ELF97 fosfát je hydrolyzován enzymem fosfatázou přičemž vzniká fluorescenční nerozpustný produkt ELF 97 alkohol (ELFA). Ve fosforem limitovaných bakteriálních kulturách bylo vysoké procento buněk značeno ELFA (26–100% v závislosti na kmenu). Naproti tomu ve vzorcích ze Středozemního moře to bylo jen 0,01%, přesto že byla prokázána limitace fosforem. (cs)
  • The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor.
  • The fluorogenic substrate Enzyme-Labeled Fluorescence 97 (ELF-P) is hydrolyzed by the P-cleaving enzyme phosphatase, producing ELF 97 alcohol (ELFA), a fluorescent-insoluble product. A high percentage of the cultured cells were labeled with ELFA (the ratio of ELFA spots to total DAPI counts in P-limited cultures ranged from 26 to 100%, depending on the strain). In contrast, this ratio never exceeded 0.01% in Mediterranean samples, even when P was demonstrated to be a significant limiting factor. (en)
Title
  • Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea
  • Aktivita alkalických fosfatáz mořských bakterií studovaná pomocí substrátu ELF97: úspěchy a meze použitelnosti metody ve fosforem limitovaném Středozemním moři (cs)
  • Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea (en)
skos:prefLabel
  • Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea
  • Aktivita alkalických fosfatáz mořských bakterií studovaná pomocí substrátu ELF97: úspěchy a meze použitelnosti metody ve fosforem limitovaném Středozemním moři (cs)
  • Alkaline phosphatase activity of marine bacteria studied with ELF 97 substrate: success and limits in the P-limited Mediterranean Sea (en)
skos:notation
  • RIV/60077344:_____/08:00317620!RIV09-AV0-60077344
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z60170517)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 355428
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/08:00317620
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • marine bacteria; alkaline phosphatase; ELF97 phosphatase substrate (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [1FB021582298]
http://linked.open...i/riv/nazevZdroje
  • Aquatic Microbial Ecology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 52
http://linked.open...iv/tvurceVysledku
  • Nedoma, Jiří
  • Van Wambeke, F.
  • Duhamel, S.
  • Lebaron, P.
http://linked.open...ain/vavai/riv/wos
  • 000259980500004
http://linked.open...n/vavai/riv/zamer
issn
  • 0948-3055
number of pages
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