| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Atenuovaný kmen (263) viru klíšťové encefalitidy izolovaný z klíšťat byl jednak pasážován 5x v laboratorních myších, jednak při 40°C v PS buňkách. Vznikly dva nezávislé kmeny, 263-m5 a 263-TR s identickou genomickou sekvencí. Oba kmeny vykazovaly velkou velikost plaků v buněčné kultuře, neuroinvazivitu a teplotní rezistenci. Sekvenční analýza odhalila dvě unikátní aminokyselinové substituce, jedna byla v blízkosti katalytického místa virové proteázy. Pozorování navrhují, že při adaptaci viru z klíšťat na savčího hostitele dochází k selekci preexcistujících virulentních variant z „quasispecies“, spíše než ke vzniku nových mutací. Význam těchto pozorování je diskutován. (cs)
- An attenuated strain (263) of the tick-borne encephalitis virus, isolated from field ticks, was either serially subcultured, 5 times in mice, or at 40 degrees C in PS cells, producing 2 independent strains, 263-m5 and 263-TR with identical genomes; both strains exhibited increased plaque size, neuroinvasiveness and temperature-resistance. Sequencing revealed two unique amino acid substitutions, one mapping close to the catalytic site of the viral protease. These observations imply that virus adaptation from ticks to mammals occurs by selection of pre-existing virulent variants from the quasispecies population rather than by the emergence of new random mutations. The significance of these observations is discussed.
- An attenuated strain (263) of the tick-borne encephalitis virus, isolated from field ticks, was either serially subcultured, 5 times in mice, or at 40 degrees C in PS cells, producing 2 independent strains, 263-m5 and 263-TR with identical genomes; both strains exhibited increased plaque size, neuroinvasiveness and temperature-resistance. Sequencing revealed two unique amino acid substitutions, one mapping close to the catalytic site of the viral protease. These observations imply that virus adaptation from ticks to mammals occurs by selection of pre-existing virulent variants from the quasispecies population rather than by the emergence of new random mutations. The significance of these observations is discussed. (en)
|
| Title
| - Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus
- Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus (en)
- Mutace v genech NS2B a NS3 ovlivňují neuroinvazivitu západoevropského kmenu viru klíšťové encefalitidy na modelu laboratorní myši (cs)
|
| skos:prefLabel
| - Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus
- Mutations in the NS2B and NS3 genes affect mouse neuroinvasiveness of a Western European field strain of tick-borne encephalitis virus (en)
- Mutace v genech NS2B a NS3 ovlivňují neuroinvazivitu západoevropského kmenu viru klíšťové encefalitidy na modelu laboratorní myši (cs)
|
| skos:notation
| - RIV/60077344:_____/08:00307825!RIV08-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA524/06/1479), P(GA524/08/1509), P(LC06009), Z(AV0Z60220518)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/08:00307825
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - tick-borne encephalitis virus; neuroinvasiveness; viral phenotype (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Golovchenko, Maryna
- Grubhoffer, Libor
- Kopecký, Jan
- Rudenko, Natalia
- Růžek, Daniel
- Gould, E. A.
- Forrester, N. L.
- Gritsun, T. S.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
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