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| rdf:type
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| Description
| - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P<0.05) between cooling regimes were observed where Mr Frosty was more variable (Inter-Quartile Range being 21.5%, versus 13.0% for CRF samples). Viability assessment using fluorescein diacetate gave significantly (P<0.0001) higher survival than growth in agar with median values being 96% and 89%, respectively.
- Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P<0.05) between cooling regimes were observed where Mr Frosty was more variable (Inter-Quartile Range being 21.5%, versus 13.0% for CRF samples). Viability assessment using fluorescein diacetate gave significantly (P<0.0001) higher survival than growth in agar with median values being 96% and 89%, respectively. (en)
- Dvě kryoprezervační metody, klasická s řízeným zmrazováním a enkapsulační-dehydratační založená na vitrifikaci, byly validovány pěti členy EU výzkumného konsorcia COBRA a dvěma nezávislými externími laboratořemi. Jako testovací organismus byl použit kmen Chlorella vulgaris SAG 211-11b. Dvoustupňové zmrazování pomocí pasivního ochlazování (Mr Frosty) vykazovalo větší variabilitu nežli pomocí přístrojově řízeného zmrazování. Testy viability pomocí fluorescein diacetátu vykazovaly vyšší procento přežívání nežli růst kolonií na agaru. Největší variabilita mezi validátory se ukázala při aplikaci enkapsulace-dehydratace. (cs)
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| Title
| - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections
- Využití fyzické a virtuální infrastruktury při validaci metod kryoprezervace řas v mezinárodních sbírkách (cs)
- The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections (en)
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| skos:prefLabel
| - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections
- Využití fyzické a virtuální infrastruktury při validaci metod kryoprezervace řas v mezinárodních sbírkách (cs)
- The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections (en)
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| skos:notation
| - RIV/60077344:_____/07:00092230!RIV08-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - V, Z(AV0Z60050516), Z(AV0Z60660521)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00092230
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - cryopreservation; storage; culture collections (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Elster, Josef
- Hrouzek, Pavel
- Lukavský, Jaromír
- Lukešová, Alena
- Lorenz, M.
- Brennan, D.
- Müller, J.
- Lukeš, Martin
- Probert, I.
- Day, J. G.
- Benson, E. E.
- Harding, K.
- Friedl, T.
- Santos, L. M. A.
- Santos, M. F.
- Amaral, R.
- Osório, H. C.
- Pröschold, T.
- Ryan, M. J.
- Wilding, T. A.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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