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| rdf:type
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| Description
| - Trávicí enzymy vířníků byly detekovány a lokalizovány pomocí ELF (Enzyme Labelled Fluorescence) metody. Fluorescenční substrát (ELF97) vytváří precipitáty ELF alkoholu (ELFA) na místě enzymatické aktivity a tím umožňuje její přímou mikroskopickou lokalizaci. Vývoj ELFA značení byl pozorován při různě dlouhých inkubacích. K detekci trávicích enzymů byla nejvhodnější doba inkubace tři hodiny. Fosfatázová, .beta.-N-acetylhexosaminidázová a lipázová aktivita byla studována ve vířnících Brachionus angularis, B. calyciflorus, Keratella cochlearis a Lecane closterocerca z fed-batch kultur. U všech studovaných druhů byla nejčastějším místem aktivity enzymů oblast žaludku a střeva a u L. closterocerca oblast mastaxu. Fosfatázy byly často lokalizovány na vířivém aparátu B. calyciflorus. (cs)
- Hydrolytic enzymes involved in rotifer digestive processes were investigated directly at the sites of enzyme action using the ELF (Enzyme Labelled Fluorescence) technique. After enzymatic hydrolysis of an artificial ELF substrate, the fluorescent product ELF alcohol (ELFA) marked the sites of enzyme action. The time development of ELFA labelling was studied at different incubation times. Phosphatases, .beta.-N-acetylhexosaminidases and lipases were examined in Brachionus angularis, B. calyciflorus, Keratella cochlearis and Lecane closterocerca from fed-batch cultures. We detected activities of all studied enzymes mostly in the stomach and intestine of rotifers. L. closterocerca was the only species showing enzyme activity at the mastax. Lipase activity was observed in the stomach and intestine of all species and in the mastax of L. closterocerca. Phosphatases were frequently located at the corona of B. calyciflorus.
- Hydrolytic enzymes involved in rotifer digestive processes were investigated directly at the sites of enzyme action using the ELF (Enzyme Labelled Fluorescence) technique. After enzymatic hydrolysis of an artificial ELF substrate, the fluorescent product ELF alcohol (ELFA) marked the sites of enzyme action. The time development of ELFA labelling was studied at different incubation times. Phosphatases, .beta.-N-acetylhexosaminidases and lipases were examined in Brachionus angularis, B. calyciflorus, Keratella cochlearis and Lecane closterocerca from fed-batch cultures. We detected activities of all studied enzymes mostly in the stomach and intestine of rotifers. L. closterocerca was the only species showing enzyme activity at the mastax. Lipase activity was observed in the stomach and intestine of all species and in the mastax of L. closterocerca. Phosphatases were frequently located at the corona of B. calyciflorus. (en)
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| Title
| - Rotifer digestive enzymes: direct detection using the ELF technique
- Trávicí enzymy vířníků: přímá detekce pomocí ELF metody (cs)
- Rotifer digestive enzymes: direct detection using the ELF technique (en)
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| skos:prefLabel
| - Rotifer digestive enzymes: direct detection using the ELF technique
- Trávicí enzymy vířníků: přímá detekce pomocí ELF metody (cs)
- Rotifer digestive enzymes: direct detection using the ELF technique (en)
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| skos:notation
| - RIV/60077344:_____/07:00088824!RIV08-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1QS600170504), P(IAA600170602), P(IAA6017202), S, Z(AV0Z60170517), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00088824
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - rotifers; enzyme localization; phosphatase; lipase; .beta.-N-acetylhexosaminidase (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Vrba, Jaroslav
- Štrojsová, M.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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