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| rdf:type
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| Description
| - NADH dehydrogenázová aktivita byla charakterizována v mitochondriálním lyzátu Phytomonas serpens, bičíkatého trypanosomatida parazitujícího rostliny. Dvě odlišné vysokomolekulární NADH dehydrogenázy byly charakterizovány nativní PAGE a detekovány přímým barvením aktivity v gelu. Asociace NADH dehydrogenázových aktivit se dvěma různými multipodjednotkovými komplexy byla odhaleny ve druhé dimenzi, provedené za denaturačních podmínek. Jedna podjednotka přítomná v obou komplexech zkříženě reagovala s protilátkou proti 39 kDa podjednotkce kravského komplexu I. Z několika podjednotek analyzovaných MS jedna obsahovala doménu charakteristickou pro rodinu LYR podjednotek NADH:ubichinon oxidoreduktáz. Spektrofotometrické měření NADH:ubichinon 10 a NADH:ferrikyanid dehydrogenázové aktivity odhalilo jejich různou citlivost k rotenonu, piericidinu a diphenyl iodoniu. (cs)
- NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens, a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity staining. The association of NADH dehydrogenase activities with two distinct multisubunit complexes was revealed in the second dimension performed under denaturing conditions. One subunit present in both complexes cross-reacted with the antibody against the 39 kDa subunit of bovine complex I. Out of several subunits analyzed by MS, one contained a domain characteristic for the LYR family subunit of the NADH:ubiquinone oxidoreductases. Spectrophotometric measurement of the NADH:ubiquinone 10 and NADH:ferricyanide dehydrogenase activities revealed their different sensitivities to rotenone, piericidin, and diphenyl iodonium.
- NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens, a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity staining. The association of NADH dehydrogenase activities with two distinct multisubunit complexes was revealed in the second dimension performed under denaturing conditions. One subunit present in both complexes cross-reacted with the antibody against the 39 kDa subunit of bovine complex I. Out of several subunits analyzed by MS, one contained a domain characteristic for the LYR family subunit of the NADH:ubiquinone oxidoreductases. Spectrophotometric measurement of the NADH:ubiquinone 10 and NADH:ferricyanide dehydrogenase activities revealed their different sensitivities to rotenone, piericidin, and diphenyl iodonium. (en)
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| Title
| - Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida)
- Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) (en)
- Charakterizace NADH:ubichinon oxidoreduktázy (komplexu I) u trypanosomatida Phytomonas serpens (Kinetoplastida) (cs)
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| skos:prefLabel
| - Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida)
- Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) (en)
- Charakterizace NADH:ubichinon oxidoreduktázy (komplexu I) u trypanosomatida Phytomonas serpens (Kinetoplastida) (cs)
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| skos:notation
| - RIV/60077344:_____/07:00087511!RIV08-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(2B06129), P(GA204/06/1558), P(LC07032), S, Z(AV0Z50200510), Z(AV0Z60220518)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00087511
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - NADH dehydrogenase; complex I; Phytomonas; respiratory chain; trypanosomatid (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Horváth, A.
- Lukeš, Julius
- Man, Petr
- Verner, Zdeněk
- Čermáková, P.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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