About: Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters. We indirectly studied the affinity of extracellular phosphatases for ELF97 phosphate (ELFP) in different aquatic environments through its ability to inhibit the hydrolysis of 4-methylumbelliferyl phosphate. Values of inhibition constants, Ki, which correspond to the concentrations necessary for half saturation of phosphatases by ELFP,were lowest in the oligotrophic Mediterranean Sea.We found higher values in oligo- to mesotrophic acidified lakes, in a eutrophic reservoir and in a pure culture of the marine bacterium Alteromonas infernus. Our experiments indicated that the assay ELFP concentration in the endogeneous phosphatase detection kit (Molecular Probes) used would ensure >99% saturation of extracellular phosphatases in marine environments and >90% saturation in the studied fresh waters. (en) Studovali jsme afinitu extracelulárních fosfatáz pro substrát ELF97 fosfát (ELFP) v různých akvatických ekosystémech nepřímou metodou založenou na inhibici hydrolýzy 4-metylumbeliferyl fosfátu. Hdnoty inhibiční konstanty, Ki, které odpovídají koncentraci potřebné pro 50-% saturaci enzymu ELFP, byly nejnižší v oligotrofní oblasti Středozemního moře, vyšší v acidifikovaných jezerech, eutrofní přehradě a v kultuře bakterie Alteromonas infernus. Naše pokusy ukazují že koncetrace ELFP v kitu na měření endogenních fosfatáz (Molecular Probes) zajišťuje >99% saturaci extracelulárních fosfatáz v moři a >90% saturaci ve sladkých vodách. (cs)
Title	Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments Afinita extracelulárních fosfatáz pro ELF97 fosfát ve vodním prostředí (cs) Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments (en)
skos:prefLabel	Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments Afinita extracelulárních fosfatáz pro ELF97 fosfát ve vodním prostředí (cs) Affinity of extracellular phosphatases for ELF97 phosphate in aquatic environments (en)
skos:notation	RIV/60077344:_____/07:00086809!RIV08-AV0-60077344
http://linked.open.../vavai/riv/strany	454;460
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(IAA600170602), P(IAA6017202), V, Z(AV0Z60170517)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Nedoma, Jiří Štrojsová, Martina Ševců, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	408715
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/07:00086809
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ELF97 phosphate; microbial phosphatases; affinity (en)
http://linked.open.../riv/klicoveSlovo	affinity ELF97 phosphate microbial phosphatases
http://linked.open...odStatuVydavatele	AU - Australské společenství
http://linked.open...ontrolniKodProRIV	[5D99F62681C2]
http://linked.open...i/riv/nazevZdroje	Marine and Freshwater Research
http://linked.open...in/vavai/riv/obor	DA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Detection, localisation and quantification of extracellular enzymatic activities on cellular and population level in natural lake seston Regulation of extracellular phosphatase activity in different morphotypes of natural bacterioplankton studied at the single-cell level
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Nedoma, Jiří Van Wambeke, F. Štrojsová, Alena Štrojsová, Martina Duhamel, S.
http://linked.open...n/vavai/riv/zamer	Struktura, funkce a vývoj vodních ekosystémů
issn	1323-1650
number of pages	7 (xsd:int)

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