About: Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation

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About: Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Gene resources include original Czech fish strains as a part of our national culture heritage (7 strains of common carp and 2 strains of European catfish). In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and European catfish. A sperm freezing procedure was developed, providing good motility of spermatozoa, fertilization and hatching rates in each of the species. Common carp sperm was collected in plastic containers (1/10 - 1/30 volume of sperm andair) and stored under aerobic condition at 4 oC for 1-3 hours. Sperm was diluted 1:5 in a Kurokura extender (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3) and equilibrated for 40 minutes at 4 oC. Diluted sperm was transferred to 2 ml cryotube s and 10 % of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 oC to -9 oC at a rate of 4 oC . min-1, then from -11 oC to -80 oC at a rate of 11 oC .min-1, hold for 6 min at -80 o Gene resources include original Czech fish strains as a part of our national culture heritage (7 strains of common carp and 2 strains of European catfish). In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and European catfish. A sperm freezing procedure was developed, providing good motility of spermatozoa, fertilization and hatching rates in each of the species. Common carp sperm was collected in plastic containers (1/10 - 1/30 volume of sperm andair) and stored under aerobic condition at 4 oC for 1-3 hours. Sperm was diluted 1:5 in a Kurokura extender (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3) and equilibrated for 40 minutes at 4 oC. Diluted sperm was transferred to 2 ml cryotube s and 10 % of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 oC to -9 oC at a rate of 4 oC . min-1, then from -11 oC to -80 oC at a rate of 11 oC .min-1, hold for 6 min at -80 o (en)
Title	Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation (en)
skos:prefLabel	Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation Cryopreservation of common carp Cyprinus carpio and European catfish Silurus glanis spermatozoa for gene resources conservation (en)
skos:notation	RIV/60076658:_____/02:00004203!RIV/2003/MSM/120003/N
http://linked.open.../vavai/riv/strany	1
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 126100001)
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Linhart, Otomar Rodina, Marek
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	641932
http://linked.open...ai/riv/idVysledku	RIV/60076658:_____/02:00004203
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	common carp, Cyprinus carpio, european catfish, Silurus glanis, cryopreservation, spermatozoa, sperm, gene resources conservation (en)
http://linked.open.../riv/klicoveSlovo	common carp Cyprinus carpio cryopreservation sperm european catfish gene resources conservation spermatozoa Silurus glanis
http://linked.open...ontrolniKodProRIV	[93764DA3CD38]
http://linked.open...v/mistoKonaniAkce	Hradec Králové
http://linked.open...i/riv/mistoVydani	Hradec Králové
http://linked.open...i/riv/nazevZdroje	International Conference Joined Meeting of the IIR with the Society for Low Temperature Biology%22CRYOPRESERVATION AND SAFE KEEPING OF CELLS AND TISSUES
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...iv/tvurceVysledku	Linhart, Otomar Rodina, Marek
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2002-05-13 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20126100001
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Society for Low Temperature Biology

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