About: Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa.

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About: Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1 then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100 % cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full period of swimming w Motility parameters of paddlefish (Polyodon spathula) spermatozoa, in different environmental conditions were investigated in this study. Paddlefish spermatozoa demonstrated the following characteristics: 100% of the cells were motile 10 s after activation with velocity 130-160 #m.s-1 then after 2 min velocity decreased to 80-130 #m.s-1; the period of motility was up to 9 minutes. Concentrations between 0.5 to 5 mM of KCl prevented activation of spermatozoa. When transferred into a swimming medium (20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined 5 mM of NaCl or MgCl2) 80-100 % cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm motility at 10, 40, 50 and 60 s after activation with stable velocity of sperm around 140 #m.s-1. Very low concentration of 0.125 mM CaCl2 combined with 0.5 mM KCl initiated motility in 20% of sperm while all sperm were activated after 2 minutes with 0.25 mM CaCl2 in similar medium for the full period of swimming w (en)
Title	Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula) (cs) Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa. (en) Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)
skos:prefLabel	Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula) (cs) Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa. (en) Vliv iontů a imobilizačních médií na motilitu čerstvého a demembranovaného spermatu veslonosa amerického (Polyodon spathula)
skos:notation	RIV/60076658:_____/02:00004199!RIV/2003/MSM/120003/N
http://linked.open.../vavai/riv/strany	326-328
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 126100001)
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Linhart, Otomar Rodina, Marek
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	668923
http://linked.open...ai/riv/idVysledku	RIV/60076658:_____/02:00004199
http://linked.open...riv/jazykVysledku	cze - čeština
http://linked.open.../riv/klicovaSlova	paddlefish, Polyodon spathula, ions, motility, demembranation, sperm, spermatozoa, (en)
http://linked.open.../riv/klicoveSlovo	ions motility sperm Polyodon spathula demembranation spermatozoa paddlefish
http://linked.open...ontrolniKodProRIV	[7B89387D40DB]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	V. Česká ichtyologická konference, Sborník referátů z vědecké konference
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...iv/tvurceVysledku	Linhart, Otomar Rodina, Marek Cosson, Jacky Mims, Steve Shelton, W. L.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20126100001
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	80-7157-614-X

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