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| rdf:type
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| Description
| - Kořeny stromů mangrove Acanthus ilicifolius, Ceriops tagal a Excoecaria agallocha sebrané v lesích Sundarbans v Indii byly obarveny trypanovou modří k pozorování kolonizace arbuskulární mykorrhizy. Spóry houby arbuskulární mykorrhizy izolované z rhizosférní půdy a sbírané společně se vzorky kořenů byly také obarveny pro testování vhodnosti barviva jako flurochromu. Bylo konstruováno vyobrazení konfokální laserovou mikroskopií. A. ilicifolius a E. agallocha projevovaly áronovitý typ kolonizace s vysoce rozvětvenými arbuskulemi, zatímco C. tagal poukazoval na seskupení typu Paris se složenými arbuskulemi v trsech. Dokázali jsme, že trypanová modř jako fluorochrom je vhodná k obarvení spór arbuskulární mykorhizy, fungální hyfy, arbuskulí a měchýřků, které mají pravděpodobně velké množství povrchového chitinu. Zdá se, že začlenění chitinu do fungální buněčné stěny a tím i jeho vhodnost jako barvivo se mění. (cs)
- Roots of three mangroves, Acanthus ilicifolius, Ceriops tagal and Excoecaria agallocha, collected from forests of the Sundarbans of India were stained with trypan blue to observe arbuscular mycorrhizal colonization. Spores of arbuscular mycorrhizal fungi isolated from rhizospheric soil, collected together with the root samples, also were stained for testing the suitability of the dye as a fluorochrome. Confocal laser scanning microscopy images were constructed. A. ilicifolius and E. agallocha exhibited Arum type colonization with highly branched arbuscules, whereas C. tagal showed Paris type association with clumped and collapsed arbuscules. We demonstrated that trypan blue is a suitable fluorochrome for staining arbuscular mycorrhizal fungal spores, fungal hyphae, arbuscules and vesicles, which presumably have a considerable amount of surface chitin. It appears that as the integration of chitin into the fungal cell wall changes, its accessibility to trypan blue dye also changes.
- Roots of three mangroves, Acanthus ilicifolius, Ceriops tagal and Excoecaria agallocha, collected from forests of the Sundarbans of India were stained with trypan blue to observe arbuscular mycorrhizal colonization. Spores of arbuscular mycorrhizal fungi isolated from rhizospheric soil, collected together with the root samples, also were stained for testing the suitability of the dye as a fluorochrome. Confocal laser scanning microscopy images were constructed. A. ilicifolius and E. agallocha exhibited Arum type colonization with highly branched arbuscules, whereas C. tagal showed Paris type association with clumped and collapsed arbuscules. We demonstrated that trypan blue is a suitable fluorochrome for staining arbuscular mycorrhizal fungal spores, fungal hyphae, arbuscules and vesicles, which presumably have a considerable amount of surface chitin. It appears that as the integration of chitin into the fungal cell wall changes, its accessibility to trypan blue dye also changes. (en)
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| Title
| - Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves
- Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves (en)
- Trypanová modř jako fluorochrom pro konfokální laserovou mikroskopii arbuskulární mykorhizy ve třech stromech mangrove (cs)
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| skos:prefLabel
| - Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves
- Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves (en)
- Trypanová modř jako fluorochrom pro konfokální laserovou mikroskopii arbuskulární mykorhizy ve třech stromech mangrove (cs)
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| skos:notation
| - RIV/60076658:12640/08:00009974!RIV09-MSM-12640___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12640/08:00009974
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - acanthus ilicifolius; arbuscular mycorrhizal fungal spores; arbuscular mycorrhizae; ceriops tagal; confocal microscopy; excoecaria agallocha; fluorescence; trypan blue (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biotechnic& Histochemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Sarafis, Vassilios
- Kumar, T.
- Das, P.
- Ghose, M.
- Majumdar, A.
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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