| Attributes | Values |
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| rdf:type
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| Description
| - Význam Gun4 proteinu u sinic pro metabolismus chlorofylu a skládání fotosyntetických komplexů (cs)
- Gun4 is a porphyrin-binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In plants, GUN4 has been implicated in plastid-to-nucleus retrograde signaling processes that coordinate both photosystem II and photosystem I nuclear gene expression with chloroplast function. In this work we present the functional analysis of Gun4 from the cyanobacterium Synechocystis sp. PCC 6803. Affinity co-purification of the FLAG-tagged Gun4 with the ChlH subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4 mutant strain that exhibited a decreased activity of magnesium chelatase. Consequently, the cellular content of chlorophyll-binding proteins was highly inadequate, especially that of proteins of photosystem II. Immunoblot analyses, blue native polyacrylamide gel electrophoresis, and radiolabeling of the
- Gun4 is a porphyrin-binding protein that activates magnesium chelatase, a multimeric enzyme catalyzing the first committed step in chlorophyll biosynthesis. In plants, GUN4 has been implicated in plastid-to-nucleus retrograde signaling processes that coordinate both photosystem II and photosystem I nuclear gene expression with chloroplast function. In this work we present the functional analysis of Gun4 from the cyanobacterium Synechocystis sp. PCC 6803. Affinity co-purification of the FLAG-tagged Gun4 with the ChlH subunit of the magnesium chelatase confirmed the association of Gun4 with the enzyme in cyanobacteria. Inactivation of the gun4 gene abolished photoautotrophic growth of the resulting gun4 mutant strain that exhibited a decreased activity of magnesium chelatase. Consequently, the cellular content of chlorophyll-binding proteins was highly inadequate, especially that of proteins of photosystem II. Immunoblot analyses, blue native polyacrylamide gel electrophoresis, and radiolabeling of the (en)
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| Title
| - Improtance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes
- Význam Gun4 proteinu u sinic pro metabolismus chlorofylu a skládání fotosyntetických komplexů (cs)
- Improtance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes (en)
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| skos:prefLabel
| - Improtance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes
- Význam Gun4 proteinu u sinic pro metabolismus chlorofylu a skládání fotosyntetických komplexů (cs)
- Improtance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes (en)
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| skos:notation
| - RIV/60076658:12640/08:00009367!RIV09-MSM-12640___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA500200713), Z(AV0Z50200510), Z(AV0Z50510513), Z(MSM6007665808)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12640/08:00009367
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Synechocystis sp PCC-6803; sp. PCC 6803; photosystem-II; tetrapyrrole biosynthesis; signal-transduction; gene-expression; in-vivo; mutant; light; ferrochelatase (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Biological Chemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Sobotka, Roman
- Tichý, Martin
- Komenda, Josef
- Gardian, Zdenko
- Dühring, Ulf
- Grimm, Bernhard
- Peter, Enrico
- Wilde, Annegret
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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