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rdf:type
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Description
| - Porovnávali jsme efekt dvou kmenů jednoho viru (italský a španělský kmen tobamoviru PMMoV) v hostitelské rostlině Nicotiana benthamiana pomocí zobrazovací fluorimetrie. Infekce byla detekována změnou konvenčních parametrů fluorescence chlorofylu a/nebo za pomoci pokročilé statistické metody ? kombinatoriálního imagingu. Koncenční parametry detekovaly infekci v její střední fázi (tj. 16 dní po inokulaci), zatímco kombinatoriální imaging detekoval infekci již v její rané fázi - 4 dny po inokulaci v případě virulentnějšího kmene PMMoV-S a 6 dní po inokulaci pro méně virulentní kmen PMMoV-I. (cs)
- We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the last viral strain.
- We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the last viral strain. (en)
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Title
| - Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants
- Konvenční a kombinatoriální zobrazování fluorescence chlorofylu rostlin infikovaných tobamovirem (cs)
- Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants (en)
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skos:prefLabel
| - Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants
- Konvenční a kombinatoriální zobrazování fluorescence chlorofylu rostlin infikovaných tobamovirem (cs)
- Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants (en)
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skos:notation
| - RIV/60076658:12640/08:00009358!RIV09-MSM-12640___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12640/08:00009358
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - biotic stress; Nicotiana benthamiana; non-photochemical quenching; Pepper mild mottle virus (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Nedbal, Ladislav
- Soukupová, Julie
- Pineda, M.
- Matouš, Karel
- Barón, M.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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