About: Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence     Goto   Sponge   NotDistinct   Permalink

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Description
  • Sestavování fotosyntetických jednotek u Rhodobacter sphaeroides bylo studováno pomocí analýzy kinetických změn variabilní fluorescence bakteriochlororofylu a. Sestavování fotosyntetických komplexů bylo indukováno přenesením aerobní kultury Rh. sphaeroides do semiaerobních podmínek. Analýza na povídá, že nejdříve jsou sestavována reakční centra, poté vnitřní anténa LH1, poté je aktivován elektronový transport a na závěr se sestavuje vnější světlosběrná anténa LH2. (cs)
  • The development of functional photosynthetic units in Rhodobacter sphaeroides was followed by near infra-red fast repetition rate (IRFRR) fluorescence measurements that were correlated to absorption spectroscopy, electron microscopy and pigment analyses. To induce the formation of intracytoplasmic membranes (ICM) (greening), cells grown aerobically both in batch culture and in a carbon-limited chemostat were transferred to semiaerobic conditions. In both aerobic cultures, a low level of photosynthetic complexes was observed, which were composed of the reaction center and the LH1 core antenna. Interestingly, in the batch cultures the reaction centers were essentially inactive in forward electron transfer and exhibited low photochemical yields F-v/F-M, whereas the chemostat culture displayed functional reaction centers with a rather rapid (1-2 ms) electron transfer turnover, as well as a high F-v/F-M of similar to0.8. In both cases, the transfer to semiaerobiosis resulted in rapid induction of bacterioc
  • The development of functional photosynthetic units in Rhodobacter sphaeroides was followed by near infra-red fast repetition rate (IRFRR) fluorescence measurements that were correlated to absorption spectroscopy, electron microscopy and pigment analyses. To induce the formation of intracytoplasmic membranes (ICM) (greening), cells grown aerobically both in batch culture and in a carbon-limited chemostat were transferred to semiaerobic conditions. In both aerobic cultures, a low level of photosynthetic complexes was observed, which were composed of the reaction center and the LH1 core antenna. Interestingly, in the batch cultures the reaction centers were essentially inactive in forward electron transfer and exhibited low photochemical yields F-v/F-M, whereas the chemostat culture displayed functional reaction centers with a rather rapid (1-2 ms) electron transfer turnover, as well as a high F-v/F-M of similar to0.8. In both cases, the transfer to semiaerobiosis resulted in rapid induction of bacterioc (en)
Title
  • Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence
  • Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence (en)
  • Postupné sestavování fotosyntetických jednotek u Rhodobacter sphaeroides studované pomocí Fast repetition rate analýzy variabilní fluorescence bakteriochlororofylu a. (cs)
skos:prefLabel
  • Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence
  • Sequential assembly of photosynthetic units in Rhodobacter sphaeroides as revealed by fast repetition rate analysis of variable bacteriochlorophyll a fluorescence (en)
  • Postupné sestavování fotosyntetických jednotek u Rhodobacter sphaeroides studované pomocí Fast repetition rate analýzy variabilní fluorescence bakteriochlororofylu a. (cs)
skos:notation
  • RIV/60076658:12640/05:00006488!RIV06-MSM-12640___
http://linked.open.../vavai/riv/strany
  • 220-231
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A141)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 542387
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12640/05:00006488
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • membrane development; light-harvesting complex; photosynthetic membrane; fast repetition rate fluorescence; reaction center kinetics (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [A1234C5A1B3F]
http://linked.open...i/riv/nazevZdroje
  • Biochimica et Biophysica Acta - Bioenergetics
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1706
http://linked.open...iv/tvurceVysledku
  • Koblížek, Michal
  • Hunter, C. Neil
  • Breitbart, Seth I.
  • Kolber, Zbigniew S.
  • Niederman, Robert A.
  • Ratcliffe, Emma C.
  • Shih, Joseph D.
issn
  • 0005-2728
number of pages
http://localhost/t...ganizacniJednotka
  • 12640
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