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Description
| - Glutation patří mezi intra a extracelulárně působící protektivní antioxidanty téměř výhradně pouze ve své redukované formě. Poměr mezi redukovanou a oxidovanou formou uvnitř buňky je často používán jako ukazatel buněčné toxicity. Cílem této studie bylo určit obsah obou (oxidované i redukované) forem glutationu, a obsah cysteinu ve spermatu jesetera sibiřského, jesetera malého, okouna říčního a candáta obecného, a demonstrovat rozdíly mezi zástupci chrupavčitých (jeseterovitých) a kostnatých ryb. (cs)
- Glutathione belongs to a vital intra- and extra-cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ? 8 μg ml-1; GSH 126 ? 19 μg ml-1; GSSG 49 ? 7 μg ml-1), Acipenser ruthenus (cysteine 62 ? 9 μg ml-1; GSH 768 ? 115 μg ml-1; GSSG 180 ? 16 μg ml-1), Sander lucioperca (cysteine 251 ? 38
- Glutathione belongs to a vital intra- and extra-cellular protective antioxidant and is found almost exclusively in its reduced form. The ratio between its reduced and oxidized within cells is often used as a marker of cellular toxicity. The objectives of the study were to (i) determine both the reduced (GSH) and oxidized glutathione (GSSG) and cysteine (Cys) in the sperm of the Acipenser baerii and Acipenser ruthenus, as well as in Perca fluviatilis and Sander lucioperca, and (ii) to demonstrate the differences in concentration levels between representatives of acipenseriform and teleost species. High performance liquid chromatography with electrochemical detection was employed. The average content of the thiols determined in the sperm samples were as follows: Acipenser baerii (cysteine 55 ? 8 μg ml-1; GSH 126 ? 19 μg ml-1; GSSG 49 ? 7 μg ml-1), Acipenser ruthenus (cysteine 62 ? 9 μg ml-1; GSH 768 ? 115 μg ml-1; GSSG 180 ? 16 μg ml-1), Sander lucioperca (cysteine 251 ? 38 (en)
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Title
| - Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)
- Glutation, redukovaná forma, oxidovaá forma, cystein, Acipenser baerii, Acipenser ruthenus, Perca fluviatilis, Sander lucioperca, spermatozoa (cs)
- Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca) (en)
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skos:prefLabel
| - Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca)
- Glutation, redukovaná forma, oxidovaá forma, cystein, Acipenser baerii, Acipenser ruthenus, Perca fluviatilis, Sander lucioperca, spermatozoa (cs)
- Content of cysteine, reduced and oxidized glutathione in spermatozoa of representatives of Acipenseriformes (Acipenser baerii and A. ruthenus) as well as teleosts (Perca fluviatilis and Sander lucioperca) (en)
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skos:notation
| - RIV/60076658:12610/08:00008882!RIV09-MSM-12610___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - Z(MSM6007665809), Z(MSM6215712402)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/08:00008882
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Glutathione; reduced glutathione; oxidized glutathione; cysteine; Acipenser baerii; Acipenser ruthenus; Perca fluviatilis; Sander lucioperca; spermatozoa (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Applied Ichthyology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Adam, V.
- Kizek, R.
- Rodina, Marek
- Svobodová, Zdeňka
- Stejskal, K.
- Fabrik, I.
- Beklová, M.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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