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| rdf:type
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| Description
| - The objective of this study was to freeze sperm of sex-reversed females (neomales) of perch and to test their fertilization ability. Sperm used was testicular (TSN), collected from females that have been inverted by means of externally administered 17-alpha methyltestosterone. Sperm collected from intact males (SSNM) of the same origin were used as control. Prior to freezing, both TSN and SSNM were diluted into 300 mm glucose solution at the ratio of 1 : 6 and DMSO was used as cryoprotectant (10% final concentration). Crypreservation was performed in 0.5 ml straws placed into a polystyrene box, three cm above the liquid nitrogen level for 10 min and thereafter transferred fully into liquid nitrogen. Samples were thawed in 40°C water bath for 8 s and used for the fertilization experiments. Spermatozoa concentration of fresh TSN and SSNM were estimated with 45.3 x 109 and 37.8 x 109 spermatozoa ml-1, respectively. Both sperm velocity and motility showed significant decreases in the TSN (134.6 μm s-
- The objective of this study was to freeze sperm of sex-reversed females (neomales) of perch and to test their fertilization ability. Sperm used was testicular (TSN), collected from females that have been inverted by means of externally administered 17-alpha methyltestosterone. Sperm collected from intact males (SSNM) of the same origin were used as control. Prior to freezing, both TSN and SSNM were diluted into 300 mm glucose solution at the ratio of 1 : 6 and DMSO was used as cryoprotectant (10% final concentration). Crypreservation was performed in 0.5 ml straws placed into a polystyrene box, three cm above the liquid nitrogen level for 10 min and thereafter transferred fully into liquid nitrogen. Samples were thawed in 40°C water bath for 8 s and used for the fertilization experiments. Spermatozoa concentration of fresh TSN and SSNM were estimated with 45.3 x 109 and 37.8 x 109 spermatozoa ml-1, respectively. Both sperm velocity and motility showed significant decreases in the TSN (134.6 μm s- (en)
- Předmětem této práce bylo zmrazit sperma od pohlavně invertovaných jikernaček okouna a otestovat jeho oplozovací schopnost. Cílem této práce bylo pokusit se zmrazit sperma invertovaných jikernaček okouna a otestovat jeho oplozovací schopnost po rozmrazení na jikrách. Pro experimenty bylo použito testikulární sperma jikernaček invertovaných pomocí externě podávaných hormonů (17 alfa metyltestosteronu). Jako kontrola bylo použito vytřené sperma přirozených mlíčáků stejného původu. Před mražením bylo sperma naředěno 300 mM roztokem glukózy, jako kryoprotektant byl použit DMSO finální koncentrace 10%. Vlastní zmražení bylo provedeno v polystyrénovém boxu v pejetách 0,5ml 3cm nad hladinou tekutáho dusíku po dobu 10 min, poté byly pejety přeneseny do tekutého dusíku. Vzorky byly rozmrazeny ve vodní lázni 40°C po dobu 20s a použity k oplození jiker. Ukazatelem úspěšnosti kryokonzervace byla dosažená láhnivost plůdku. Líhnivost plůdku dosažená rozmrazeným neomale spermatem v dávce 12x105 SPZ na jikru byla níz (cs)
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| Title
| - Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis)
- Motilita a oplozeníschopnost zmrazených spermií normálnách mlíčáků (XY) a sex-invertovaných gynogenetických jikernaček (XX) okouna říčního (Perca fluviatilis) (cs)
- Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis) (en)
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| skos:prefLabel
| - Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis)
- Motilita a oplozeníschopnost zmrazených spermií normálnách mlíčáků (XY) a sex-invertovaných gynogenetických jikernaček (XX) okouna říčního (Perca fluviatilis) (cs)
- Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis) (en)
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| skos:notation
| - RIV/60076658:12610/08:00008881!RIV09-GA0-12610___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/0817), Z(MSM6007665809)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/08:00008881
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Perch; Perca fluviatlis; sperm; cryopreservation; neomales; sex-reversed females (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Applied Ichthyology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Linhart, Otomar
- Rodina, Marek
- Policar, Tomáš
- Rougeot, C.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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