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| Description
| - Trypsin-like activity, similar to acrosin, is present in sturgeon spermatozoa and can be a potential target for trypsin inhibitors. The objective of this work was to use a fluorescent soybean trypsin inhibitor (SBTI) conjugate with the Alexa Fluor? 488 dye for visualization of sturgeon acrosome. After incubation with SBTI-Alexa, a strong signal was observed both in the acrosome and midpiece or implantation fossa regions. We have also found that SBTI-Alexa staining can be combined with live/dead staining. Close examination of staining pattern revealed that SBTI-Alexa can either stain acrosome or whole sperm. Staining of whole sperm correlated with live/dead staining (r2 = 0.94, p < 0.01). However, in fresh semen most cells (93 - 97%) were not stained with SBTI-Alexa, probably due to intact acrosomes. Further studies should test if SBTI-Alexa can be applied to monitor the acrosome status during the acrosome reaction and cryopreservation.
- Trypsin-like activity, similar to acrosin, is present in sturgeon spermatozoa and can be a potential target for trypsin inhibitors. The objective of this work was to use a fluorescent soybean trypsin inhibitor (SBTI) conjugate with the Alexa Fluor? 488 dye for visualization of sturgeon acrosome. After incubation with SBTI-Alexa, a strong signal was observed both in the acrosome and midpiece or implantation fossa regions. We have also found that SBTI-Alexa staining can be combined with live/dead staining. Close examination of staining pattern revealed that SBTI-Alexa can either stain acrosome or whole sperm. Staining of whole sperm correlated with live/dead staining (r2 = 0.94, p < 0.01). However, in fresh semen most cells (93 - 97%) were not stained with SBTI-Alexa, probably due to intact acrosomes. Further studies should test if SBTI-Alexa can be applied to monitor the acrosome status during the acrosome reaction and cryopreservation. (en)
- Aktivita trypsinu, podobně jako akrosinu, je přítomna ve spermiích jeseterů a může být potencionálním cílem pro inhibitory trypsinu. Cíl této práce bylo užití Soybean Trypsin Inhibitoru (SBTI) spojeným s Alexa Fluor? 488 fluorescenční barvy (Alexa) pro vizualizaci akrosomu. Po inkubaci Alexou byl pozorovaný silný signál v akrosomu a středním oddílu či implantační jamce. Také jsme zjistili, že Alexa může být kombinována s live/dead staining. Bližším pozorováním modelového barvení jsme odhalili, že Alexa může značit jak akrosomu tak celou spermii. Barvení celých spermií korelovalo s live/dead barvením (r2 = 0.94, p < 0.01). Nicméně, u čerstvých vzorků většina spermií (93 - 97%) barvena Alexou nebyla, pravděpodobně kvůli neporušeným akrosomům. Další studie by měli testovat zda-li může být Alexa užita pro monitorování stavu akrosomu během akrosomové reakce a kryokonzervace. (cs)
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| Title
| - Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome
- Značení jeseteřích spermií pomocí Trypsin Inhibitor from Soybean, Alexa Fluor? 488 Conjugate pro vizualizaci akrosomu jesetera (cs)
- Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome (en)
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| skos:prefLabel
| - Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome
- Značení jeseteřích spermií pomocí Trypsin Inhibitor from Soybean, Alexa Fluor? 488 Conjugate pro vizualizaci akrosomu jesetera (cs)
- Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome (en)
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| skos:notation
| - RIV/60076658:12610/08:00008879!RIV09-AV0-12610___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/0817), P(IAA608030801), Z(MSM6007665809)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/08:00008879
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - SBTI-Alexa; sturgeon; acrosome; spermatozoa (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Applied Ichthyology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Alavi, Sayyed Mohammad Hadi
- Gela, David
- Kašpar, Vojtěch
- Linhart, Otomar
- Pšenička, Martin
- Rodina, Marek
- Cosson, Jacky
- Ciereszko, A.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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