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| Description
| - In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact.
- In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact. (en)
- V této práci jsme popsali značení akrosomu a charakteristiku motility čerstvých a kryopreservovaných spermií jesetera malého (Acipenser ruthenus L.) pomocí Soybean Trypsin Inhibitor-Alexa Conjugate Fluorescent Staining a systému CASA (computer-aided sperm analysis). Pro kryopreservaci byl použit metanol a dimethylsulfoxid (DMSO). Po kryopreservaci došlo k typickému poklesu motility spermií, ovšem bez rozdílů mezi kryoprotektanty. Kryopreservace způsobovala zvýšení procenta spermií se značeným akrosomem pomocí SBTI-Alexa po použití DMSO ve srovnání s metanolem. Tyto data naznačují, že nízká vhodnost užití DMSO pro kryopreservaci jeseteřích spermií souvisí s negativním specifickým účinkem na akrosom, způsobený pravděpodobně jeho předčasným spuštěním, ještě před kontaktem s jikrou. (cs)
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| Title
| - Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO
- Značení akrosomu a charakteristika motility spermií jesetera malého po kryopreservaci s užitím metanolu a DMSO (cs)
- Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO (en)
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| skos:prefLabel
| - Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO
- Značení akrosomu a charakteristika motility spermií jesetera malého po kryopreservaci s užitím metanolu a DMSO (cs)
- Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO (en)
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| skos:notation
| - RIV/60076658:12610/08:00008873!RIV09-GA0-12610___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/0817), Z(MSM6007665809)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/08:00008873
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Sturgeon; Spermatozoa; Acrosome; Cryopreservation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Linhart, Otomar
- Pšenička, Martin
- Rodina, Marek
- Ciereszko, A.
- Dietrich, G. J.
- Cosson, J.
- Nynca, J.
- Wojtczak, M.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| http://localhost/t...ganizacniJednotka
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