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| - In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4°C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4°C to -9°C at a rate of 4°C . min-1, then from -9°C to -80°C at a rate of 11°C . min-1, held for 6 min at -80°C), or a rapid cooling programme L1 (from +4°C to -80°C at a rate of 20°C . min-1, held for 6 min at -80°C) and finally transferred into liquid N2. The spermatozoa were thwed in water bath 40°C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spermatozoa from vi
- In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian tench during three experiments. Sperm was collected directly into immobilizing solution of Kurokura and equilibrated at 4°C for 2,5 h. Collected sperm was transferred to 2 ml cryotubes or straws (0.5, 1 and 5 ml) and and cryoprotectants as DMSO or mixture of DMSO with propandiol were added. Than the cryotubes /straws were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled ether using a long cooling programme K4 (from +4°C to -9°C at a rate of 4°C . min-1, then from -9°C to -80°C at a rate of 11°C . min-1, held for 6 min at -80°C), or a rapid cooling programme L1 (from +4°C to -80°C at a rate of 20°C . min-1, held for 6 min at -80°C) and finally transferred into liquid N2. The spermatozoa were thwed in water bath 40°C according to the frozen volume and checked for fertilization and hatching rates. Sperm motility was evaluated for the percentage and velocity of motile spermatozoa from vi (en)
- V táto práci byla zpracovávána metoda kryokonzervace spermatu lína obecného pro potřeby ochrany genofondu. Sperma bylo odebíráno přímo do Kurokurova imobilizačního roztoku a uchováno při +4°C 2,5 hodiny. Ke spermatu byl přidán kryoprotektant, sperma bylo naplněno do 2 ml kryozkumavek nebo pejet (o objemu 0,5, 1 a 5 ml) a poté byly kryozkumavky a pejety přeneseny do předprogramovaného zmrazovacího automatu PLANER Kryo 10 serie III a mraženy delším zmrazovacím programem K4 (z teploty +4°C na -9°C rychlostí 4°C za minutu, z teploty -9°C na teplotu -80°C rychlostí 11°C za minutu, výdrž na -80°C 6 min) nebo rychlejším programem L1 ( z +4°C na -80°C rychlostí 20°C za minutu, výdrž na -80°C 6 min). Potom byly dávky přeneseny do tekutého dusíku. Sperma bylo rozmrazováno na vodní lázni teploty 40°C po dobu podle objemu dávky a použito pro test fertility na jikrách. Motilita a rychlost pohybu spermií byla zjišťována z videozáznamu mikroskopického obrazu pohybu spermií. ANOVA ve všech případech vliv zmražování. (cs)
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| Title
| - Imobilizing and cryopreservation of tench (Tinca tinca) sperm
- Imobilizace a kryokonzervace spermatu lína (Tinca tinca L.) (cs)
- Imobilizing and cryopreservation of tench (Tinca tinca) sperm (en)
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| skos:prefLabel
| - Imobilizing and cryopreservation of tench (Tinca tinca) sperm
- Imobilizace a kryokonzervace spermatu lína (Tinca tinca L.) (cs)
- Imobilizing and cryopreservation of tench (Tinca tinca) sperm (en)
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| skos:notation
| - RIV/60076658:12610/07:00007840!RIV08-GA0-12610___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/07:00007840
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Cryopreservation; hatching rate; Tench Tinca tinca; sperm motility (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Gela, David
- Linhart, Otomar
- Rodina, Marek
- Kocour, Martin
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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