| Attributes | Values |
|---|
| rdf:type
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| Description
| - Pro účely zmrazování spermatu veslonosa amerického bylo využíváno sperma s minimálně 80 % pohyblivostí. Různé procento čistého kryoprotektoru (metanol nebo DMSO) bylo přidáváno k extendoru obsahující 20 mM trisu pH 8 + 30 mM sacharózy + 0.5 mM KCl, při ředění se spermatem 1:1). Vždy 1 ml ředěného spermatu byl pipetován do 2-ml kryo-zkumavek. Kryo-zkumavky se umístily do předem na programovaného zmrazovacího automatu PLANER Kryo 10 série III s výchozí teplotou 0 oC a zmrzováno z 0 oC do -5 oC rychlostí 3 oC.min-1, z -5 oC do -15 oC rychlostí 5 oC.min-1, z -15 oC do -25 oC rychlostí 10 oC .min-1, z -25 oC do -80 oC rychlostí 20 oC .min-1, následně uchováváno po dobu 5 min při -80 oC a finálně přeneseno do kapalného dusíku. Zmrazené sperma se rozmrazovalo při 40 oC po dobu 105 s. Procento motility u rozmrazených spermií 20 s po aktivaci bylo signifikantně nižší než u čerstvého spermatu. Nejlepší kryoprotektant se jevil 8 % methanol s úrovní oplozenosti 61-76 % při použití 3,6.105 spermií na jednu jikr (cs)
- A sperm cryopreservation method using different cryoprotectants and sperm from different males was developed. Different percent of pure cryoprotectant (methanol and DMSO) was added in extender 1 or 2 (20 mM tris pH 8 + 30 mM sucrose + 0.5 mM KCl or 20 mM tris pH 8 + 50 mM sucrose + 0.5 mM KCl, dilution 1:1) or non-extended sperm and every 1 ml of mixture was transferred to a 2-ml cryotube. The cryotubes were directly transferred to a pre-programmed PLANER Kryo 10 series III at 0 oC and cooled from 0 oC to -5 oC at a rate of 3 oC.min-1, from -5 oC to -15 oC at a rate of 5 oC.min-1, from -15 oC to -25 oC at a rate of 10 oC .min-1, from -25 oC to -80 oC at a rate of 20 oC .min-1, then held for 5 min at -80 oC and finally transferred into LN2 until next morning. The sperm was thawed in a water bath at 40 oC for 105 s. Fertilization rate of control sperm (unfrozen) samples after 24-h storage at 3 oC was 81.5 % it indicated that the gametes were of good quality. Percentage and the velocity of motile sperm
- A sperm cryopreservation method using different cryoprotectants and sperm from different males was developed. Different percent of pure cryoprotectant (methanol and DMSO) was added in extender 1 or 2 (20 mM tris pH 8 + 30 mM sucrose + 0.5 mM KCl or 20 mM tris pH 8 + 50 mM sucrose + 0.5 mM KCl, dilution 1:1) or non-extended sperm and every 1 ml of mixture was transferred to a 2-ml cryotube. The cryotubes were directly transferred to a pre-programmed PLANER Kryo 10 series III at 0 oC and cooled from 0 oC to -5 oC at a rate of 3 oC.min-1, from -5 oC to -15 oC at a rate of 5 oC.min-1, from -15 oC to -25 oC at a rate of 10 oC .min-1, from -25 oC to -80 oC at a rate of 20 oC .min-1, then held for 5 min at -80 oC and finally transferred into LN2 until next morning. The sperm was thawed in a water bath at 40 oC for 105 s. Fertilization rate of control sperm (unfrozen) samples after 24-h storage at 3 oC was 81.5 % it indicated that the gametes were of good quality. Percentage and the velocity of motile sperm (en)
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| Title
| - Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa
- Vliv kryoprotektantů a samců na parametry motility ??? (cs)
- Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa (en)
|
| skos:prefLabel
| - Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa
- Vliv kryoprotektantů a samců na parametry motility ??? (cs)
- Effects of cryoprotectants and male on motility parameters and fertilizabilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa (en)
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| skos:notation
| - RIV/60076658:12610/06:00006946!RIV07-GA0-12610___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA524/03/0178), Z(MSM6007665809)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/06:00006946
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - akvakultura; ryby; motilita; reprodukce; spermatozoa; kryoprezervace; Polyodon spathula (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Journal of Applied Ichthyology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Linhart, Otomar
- Rodina, M.
- Cosson, J.
- Mims, S. D.
- Gomelsky, B.
- Cvetkova, L. I.
- Horvath, A.
- Urbanyi, B.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
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