About: Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm

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About: Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Concentrations between 0.5 to 5 mM of KCl in the swimming medium (SM) prevented activation of spermatozoa. When transferred into a SM (SM = 20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined with 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm at 10 - 60 seconds after activation up to 140 #m.s-1. Very low concentration CaCl2 (0.125 mM) combined with 0.5 mM KCl allowed initiate motility in 20% of sperm while 100 % sperm were activated at 2 minutes with 0.25 mM CaCl2 in SM. We also demonstrate that potassium (5-15 mM) inhibited demembranated sperm. Thus, movement initiation of paddlefish spermatozoa is under antagonistic control of K+ and Ca2+ ions concentration. The osmolality of seminal fluid was ranging 33.0 - 62.7 mOsmol.kg-1. The highest ionic concentration of 11.11 mmol.l-1 in seminal fluid was observed for Na+ ion. Then other concentrations declined on 1.56, 0.52 and 0.36 mmol.l-1 for K+, Ca2+ and Mg2+ Concentrations between 0.5 to 5 mM of KCl in the swimming medium (SM) prevented activation of spermatozoa. When transferred into a SM (SM = 20 mM tris, pH 8.2 and 1 mg/ml BSA) with 0.5 mM KCl (combined with 5 mM of NaCl or MgCl2) 80-100% cells were motile with velocity around 120-150 #m.s-1. MgCl2 significantly improved velocity of sperm at 10 - 60 seconds after activation up to 140 #m.s-1. Very low concentration CaCl2 (0.125 mM) combined with 0.5 mM KCl allowed initiate motility in 20% of sperm while 100 % sperm were activated at 2 minutes with 0.25 mM CaCl2 in SM. We also demonstrate that potassium (5-15 mM) inhibited demembranated sperm. Thus, movement initiation of paddlefish spermatozoa is under antagonistic control of K+ and Ca2+ ions concentration. The osmolality of seminal fluid was ranging 33.0 - 62.7 mOsmol.kg-1. The highest ionic concentration of 11.11 mmol.l-1 in seminal fluid was observed for Na+ ion. Then other concentrations declined on 1.56, 0.52 and 0.36 mmol.l-1 for K+, Ca2+ and Mg2+ (en)
Title	Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm (en)
skos:prefLabel	Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm Seminal fluid composition of paddlefish (Polyodn spathula) and ions effects on motility of fresh and demembranate sperm (en)
skos:notation	RIV/60076658:12610/03:00004986!RIV/2004/GA0/126104/N
http://linked.open.../vavai/riv/strany	54
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA524/03/0178), Z(MSM 126100001)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Linhart, Otomar Gela, David Rodina, Marek
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Výzkumný ústav rybářský a hydrobiologický
http://linked.open...dnocenehoVysledku	626688
http://linked.open...ai/riv/idVysledku	RIV/60076658:12610/03:00004986
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ions, motility, demembranate spermatozoa, common carp, Cyprinus carpio, paddlefish, Polyodon spathula (en)
http://linked.open.../riv/klicoveSlovo	ions common carp Cyprinus carpio motility Polyodon spathula paddlefish demembranate spermatozoa
http://linked.open...ontrolniKodProRIV	[5D7D82BF39C1]
http://linked.open...v/mistoKonaniAkce	Mie
http://linked.open...i/riv/mistoVydani	Mie
http://linked.open...i/riv/nazevZdroje	Book of abstracts. Genetics in Aquaculture VIII
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	A detailed study of spermatozoa in model species of chondrostean and teleostean fish
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Gela, David Linhart, Otomar Rodina, Marek Cosson, Jacky Mims, Steve Shelton, W. L.
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20126100001
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	International Association for Genetics in Aquaculture
http://localhost/t...ganizacniJednotka	12610

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