About: Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos

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About: Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, fish sperm cryopreservation methods were elaborated for ex situ conservation of 9 strains of Bohemian common carp. Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis. The percentage and velocity of sperm motility at 15 sec after activation of frozen/thawed sperm was significantly lower than that of fresh sperm (9 males). ANOVA showed a significant influence of fresh vs. frozen/thawed sperm on fertilization rate (P<0.0001), but differences In this study, fish sperm cryopreservation methods were elaborated for ex situ conservation of 9 strains of Bohemian common carp. Common carp sperm were diluted in Kurokura medium, chilled to 4 oC and dimethylsulfoxide (Me2SO) was added. Cryotubes of sperm with media were then cooled from +4 oC to -9 oC at a rate of 4 oC.min-1, then from -9 oC to -80 oC at a rate of 11 oC.min-1, held for 6 min at -80 oC and finally transferred into liquid N2. The spermatozoa were thawed in a water bath at 35 oC for 110 s and checked for fertilization yield, hatching yield of embryos and larval malformations. Fresh and frozen/thawed sperm were evaluated for the percentage and for the velocity of motile sperm from video frames using image analysis. The percentage and velocity of sperm motility at 15 sec after activation of frozen/thawed sperm was significantly lower than that of fresh sperm (9 males). ANOVA showed a significant influence of fresh vs. frozen/thawed sperm on fertilization rate (P<0.0001), but differences (en)
Title	Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos (en)
skos:prefLabel	Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos (en)
skos:notation	RIV/60076658:12610/00:00002420!RIV/2001/MSM/126101/N
http://linked.open.../vavai/riv/strany	241
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 126100001)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2001
http://linked.open...aciTvurceVysledku	Linhart, Otomar
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Výzkumný ústav rybářský a hydrobiologický
http://linked.open...dnocenehoVysledku	707757
http://linked.open...ai/riv/idVysledku	RIV/60076658:12610/00:00002420
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fish, common carp Cyprinus carpio, sperm, motility, velocity, hatching, cryopreservation (en)
http://linked.open.../riv/klicoveSlovo	hatching cryopreservation fish motility sperm velocity common carp Cyprinus carpio
http://linked.open...ontrolniKodProRIV	[FE4F11A59954]
http://linked.open...i/riv/nazevZdroje	Cryobiology
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Linhart, Otomar Rodina, M. Cosson, J.
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20126100001
issn	0011-2240
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	12610

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