About: Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes

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About: Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0105292
Description	Current standard methods for kinetic and genomic modeling cannot provide deep insight into metabolic regulation. Here, we developed and evaluated a multi-scale kinetic modeling approach applicable to any prokaryote. Specifically, we highlight the primary metabolism of the cyanobacterium Synechococcus elongatus PCC 7942. The model bridges metabolic data sets from cells grown at different CO2 conditions by integrating transcriptomic data and isozymes. Identification of the regulatory roles of isozymes allowed the calculation and explanation of the absolute metabolic concentration of 3-phosphoglycerate. To demonstrate that this method can characterize any isozyme, we determined the function of two glycolytic glyceraldehyde-3-phosphate dehydrogenases: one co-regulates high concentrations of the 3-phosphoglycerate, the other shifts the bifurcation point in hexose regulation, and both improve biomass production. Moreover, the regulatory roles of multiple phosphoglycolate phosphatases were defined for varying (non-steady) CO2 conditions, suggesting their protective role against toxic photorespiratory intermediates. Current standard methods for kinetic and genomic modeling cannot provide deep insight into metabolic regulation. Here, we developed and evaluated a multi-scale kinetic modeling approach applicable to any prokaryote. Specifically, we highlight the primary metabolism of the cyanobacterium Synechococcus elongatus PCC 7942. The model bridges metabolic data sets from cells grown at different CO2 conditions by integrating transcriptomic data and isozymes. Identification of the regulatory roles of isozymes allowed the calculation and explanation of the absolute metabolic concentration of 3-phosphoglycerate. To demonstrate that this method can characterize any isozyme, we determined the function of two glycolytic glyceraldehyde-3-phosphate dehydrogenases: one co-regulates high concentrations of the 3-phosphoglycerate, the other shifts the bifurcation point in hexose regulation, and both improve biomass production. Moreover, the regulatory roles of multiple phosphoglycolate phosphatases were defined for varying (non-steady) CO2 conditions, suggesting their protective role against toxic photorespiratory intermediates. (en)
Title	Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes (en)
skos:prefLabel	Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes Multi-Level Kinetic Model Explaining Diverse Roles of Isozymes in Prokaryotes (en)
skos:notation	RIV/60076658:12520/14:43886906!RIV15-MSM-12520___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(EE2.3.30.0006), P(LO1205)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Jablonský, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	31030
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/14:43886906
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	inactivation; pcc; gene; growth; metabolism; cyanobacteria; low co2; systems biology; synechocystis sp pcc-6803; inorganic carbon limitation (en)
http://linked.open.../riv/klicoveSlovo	inorganic carbon limitation low co2 pcc synechocystis sp pcc-6803 systems biology cyanobacteria gene growth metabolism inactivation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C4FB557124BA]
http://linked.open...i/riv/nazevZdroje	PLoS One
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	The Creation of Postdoc Positions at the University of South Bohemia and the Support of Itnersectoral Mobility by Expert Stays at the Foreign Leading R&D Institutions South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Sustainability and Excellence of the Centre of Aquaculture and Biodiversity of Hydrocenoses
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Jablonský, Jiří Hagemann, Martin Schwarz, Doreen
http://linked.open...ain/vavai/riv/wos	000340879300102
issn	1932-6203
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0105292
http://localhost/t...ganizacniJednotka	12520

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