About: Microscope point spread function, focus and calculation of optimal microscope set-up

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.tandfonline.com/doi/abs/10.1080/00207160.2013.851379#.VKqbbntlw80
Description	If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. We want to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus. If we are observing very small objects in the microscope the information appears somehow distorted. This distortion is called point spread function (PSF) and it can be measured and simulated as we show in this article. Many experiments and simulations were done in the field of confocal or deconvolution microscopy, but we want to present 3D reconstruction and measurement of PSF in the bright field (or phase contrast) microscopy. Our goal is to use this knowledge about PSF to estimate objective focus. We want to use this method for automatic focusing of the microscope. We measured the PSF on our microscope in bright field using a 100xobjective. We used 15 nm gold and 200 nm latex particles as point sources. We used two different simulations; first one was Airy disc simulation; the second one was Extended Nijboer-Zernike theory. Both approaches were used for description of PSF and finding the position of the focus. (en)
Title	Microscope point spread function, focus and calculation of optimal microscope set-up Microscope point spread function, focus and calculation of optimal microscope set-up (en)
skos:prefLabel	Microscope point spread function, focus and calculation of optimal microscope set-up Microscope point spread function, focus and calculation of optimal microscope set-up (en)
skos:notation	RIV/60076658:12520/14:43886876!RIV15-MSM-12520___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), S
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Štys, Dalibor Náhlík, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	29220
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/14:43886876
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Airy disc; bright field microscopy; point spread function; focus; ENZ theory (en)
http://linked.open.../riv/klicoveSlovo	Airy disc ENZ theory bright field microscopy point spread function focus
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[0F274F745303]
http://linked.open...i/riv/nazevZdroje	INTERNATIONAL JOURNAL OF COMPUTER MATHEMATICS
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	91
http://linked.open...iv/tvurceVysledku	Náhlík, Tomáš Štys, Dalibor
http://linked.open...ain/vavai/riv/wos	000335089900006
issn	0020-7160
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1080/00207160.2013.851379
http://localhost/t...ganizacniJednotka	12520

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