About: Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore     Goto   Sponge   NotDistinct   Permalink

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Description
  • Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization. (C) 2014 The Authors. Published by Elsevier B.V.
  • Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, and Mascot search. Forty-one and one-hundred-fifty proteins were identified in egg and spermatophore, respectively. The proteins were classified into nine categories including cell defence, cell signaling, cytoskeleton, DNA related activity, metabolism and energy production, protease and protease inhibitor, respiration, transportation, and others and unknown. Twenty-two proteins were found in both egg and spermatophore. The respiration and cytoskeleton groups are the most diverse categories in the protein profiles of the egg and spermatophore, respectively. No protein was assigned to DNA related activity and cell defence categories in the protein profile of the crayfish egg. Differences between protein profiles of the crayfish egg and spermatophore show different functional priorities for each of gametes. Several proteins having possible roles in gametogenesis, capacitation, acrosome reaction, and fertilization were identified. This proteomic profile of signal crayfish gametes provides a basis for further investigation of functional roles of the identified proteins in aspects of reproduction such as capacitation and fertilization. (C) 2014 The Authors. Published by Elsevier B.V. (en)
Title
  • Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore
  • Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore (en)
skos:prefLabel
  • Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore
  • Proteomic profiling of the signal crayfish Pacifastacus leniusculus egg and spermatophore (en)
skos:notation
  • RIV/60076658:12520/14:43886859!RIV15-GA0-12520___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/01.0024), P(EE2.3.20.0024), P(GPP502/12/P177), P(LO1205), S
http://linked.open...iv/cisloPeriodika
  • 3-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 40492
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12520/14:43886859
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Respiration; Protease; Cell signaling; Cell defence; Arthropod (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [8EDE7C68677B]
http://linked.open...i/riv/nazevZdroje
  • Animal Reproduction Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 149
http://linked.open...iv/tvurceVysledku
  • Kouba, Antonín
  • Kozák, Pavel
  • Niksirat Hashjin, Hamid
  • Andersson, Liselotte
  • James, Peter
http://linked.open...ain/vavai/riv/wos
  • 000343620200032
issn
  • 0378-4320
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.anireprosci.2014.07.024
http://localhost/t...ganizacniJednotka
  • 12520
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