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Description
  • Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software (http://rsbweb.nih.gov/ij/). We describe the plug-in's functionality, and confirm its validity for sperm head morphology analysis using fish sperm. Sperm head morphological measurements (length and width) made with the ASMA plug-in did not differ from manual measurements. Using the plug-in to measure sperm head-shaped objects of known size, the associated plug-in error rate was { 0.5%. Brightness and contrast ratios influenced sperm head measurements, suggesting the need for standardized protocols. This plug-in was effective at measuring elliptical (i.e., Atlantic cod) as well as slightly irregular (i.e., Chinook salmon) shaped sperm heads. In conclusion, our ASMA plug-in represents a versatile alternative to costly sperm morphology software.
  • Sperm head morphology has been identified as a characteristic that can be used to predict a male's semen quality. In the present study, we have developed an automated sperm head morphology analysis (ASMA) plug-in for open-source ImageJ software (http://rsbweb.nih.gov/ij/). We describe the plug-in's functionality, and confirm its validity for sperm head morphology analysis using fish sperm. Sperm head morphological measurements (length and width) made with the ASMA plug-in did not differ from manual measurements. Using the plug-in to measure sperm head-shaped objects of known size, the associated plug-in error rate was { 0.5%. Brightness and contrast ratios influenced sperm head measurements, suggesting the need for standardized protocols. This plug-in was effective at measuring elliptical (i.e., Atlantic cod) as well as slightly irregular (i.e., Chinook salmon) shaped sperm heads. In conclusion, our ASMA plug-in represents a versatile alternative to costly sperm morphology software. (en)
Title
  • Automated sperm head morphology analyzer for open-source software
  • Automated sperm head morphology analyzer for open-source software (en)
skos:prefLabel
  • Automated sperm head morphology analyzer for open-source software
  • Automated sperm head morphology analyzer for open-source software (en)
skos:notation
  • RIV/60076658:12520/11:43881801!RIV12-GA0-12520___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/01.0024), P(GA523/09/1793), S
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 187549
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12520/11:43881801
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Reproductive biology; Atlantic cod; Sperm competition; Automated Sperm Morphology Analysis; Sperm head; Sperm morphology (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [DC8BA6761D71]
http://linked.open...i/riv/nazevZdroje
  • Theriogenology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 76
http://linked.open...iv/tvurceVysledku
  • Alavi, Sayyed Mohammad Hadi
  • Butts, I.A.E.
  • Litvak, M. K.
  • Pitcher, P. E.
  • Rideout, R. M.
  • Trippel, E. A.
  • Ward, M. A. R.
http://linked.open...ain/vavai/riv/wos
  • 000297232900022
issn
  • 0093-691X
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.theriogenology.2011.06.019
http://localhost/t...ganizacniJednotka
  • 12520
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