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  • Sturgeon gametes are unique in that their spermatozoa possess acrosome whereas their eggs are perforated by numerous micropyles. Thus the function of acrosome is unclear. The acrosomal reaction of Acipenser ruthenus spermatozoa was induced by solution containing Ca2+ and high pH. The rate of affected spermatozoa was evaluated using Soybean Trypsin Inhibitor-Alexa conjugate fluorescent staining. Fertilization and hatching test proofed necessity of acrosome integrity for fertilization. Increasing percentage of spermatozoa with disrupted acrosome highly decreased percentage of hatching, in spite of electron microscopy verification that the spermatozoa bearing such disrupted acrosomes were able to reach the micropyle. In addition the significant positive correlation between the ability of spermatozoa to induce acrosomal reaction by activation solution and sperm fertilizing ability suggested that spermatozoa, which are able to activate the acrosomal reaction, are more successful in fertilization.
  • Sturgeon gametes are unique in that their spermatozoa possess acrosome whereas their eggs are perforated by numerous micropyles. Thus the function of acrosome is unclear. The acrosomal reaction of Acipenser ruthenus spermatozoa was induced by solution containing Ca2+ and high pH. The rate of affected spermatozoa was evaluated using Soybean Trypsin Inhibitor-Alexa conjugate fluorescent staining. Fertilization and hatching test proofed necessity of acrosome integrity for fertilization. Increasing percentage of spermatozoa with disrupted acrosome highly decreased percentage of hatching, in spite of electron microscopy verification that the spermatozoa bearing such disrupted acrosomes were able to reach the micropyle. In addition the significant positive correlation between the ability of spermatozoa to induce acrosomal reaction by activation solution and sperm fertilizing ability suggested that spermatozoa, which are able to activate the acrosomal reaction, are more successful in fertilization. (en)
Title
  • Potential role of the acrosome of sturgeon spermatozoa in the fertilization process
  • Potential role of the acrosome of sturgeon spermatozoa in the fertilization process (en)
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  • Potential role of the acrosome of sturgeon spermatozoa in the fertilization process
  • Potential role of the acrosome of sturgeon spermatozoa in the fertilization process (en)
skos:notation
  • RIV/60076658:12520/11:43881323!RIV12-GA0-12520___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/01.0024), P(GPP502/10/P426), P(IAA608030801), P(LC06073), P(ME10015), S, Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 222049
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12520/11:43881323
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • structure; motility; ploidy; sperm; Siberian sturgeon (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [679018551AA5]
http://linked.open...i/riv/nazevZdroje
  • Journal of Applied Ichthyology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 27
http://linked.open...iv/tvurceVysledku
  • Alavi, Sayyed Mohammad Hadi
  • Boryshpolets, Sergey
  • Gela, David
  • Kašpar, Vojtěch
  • Li, Ping
  • Linhart, Otomar
  • Pšenička, Martin
  • Rodina, Marek
  • Cosson, Jacky
  • Ciereszko, A.
http://linked.open...ain/vavai/riv/wos
  • 000288864600087
http://linked.open...n/vavai/riv/zamer
issn
  • 0175-8659
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/j.1439-0426.2010.01642.x
http://localhost/t...ganizacniJednotka
  • 12520
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