About: Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants

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About: Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of Acipenser ruthenus sperm. Four different cryoprotectans [dimethylsulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30mM sucrose, 1 mM KCl, 25 mM Tris-HCl pH 8.5) were used for the cryopreservation of sperm from five males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post-thawing motility was observed in sperm samples cryopreserved with 10% MET (46+/-19%), 10% DMA (47+/-18%) and 10% DMSO (45+/-7%). Fertilization/hatching rate in the control was 69+/-9% and 61+/-8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32+/-17%) and 5% DMA (23+/-3%) when compared to 10% DMA (9+/-5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa. The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of Acipenser ruthenus sperm. Four different cryoprotectans [dimethylsulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)] in concentrations of 5 and 10% in the extender media (30mM sucrose, 1 mM KCl, 25 mM Tris-HCl pH 8.5) were used for the cryopreservation of sperm from five males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post-thawing motility was observed in sperm samples cryopreserved with 10% MET (46+/-19%), 10% DMA (47+/-18%) and 10% DMSO (45+/-7%). Fertilization/hatching rate in the control was 69+/-9% and 61+/-8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32+/-17%) and 5% DMA (23+/-3%) when compared to 10% DMA (9+/-5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa. (en)
Title	Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants (en)
skos:prefLabel	Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants Cryopreservation of sterlet (Acipenser ruthenus) spermatozoa using different cryoprotectants (en)
skos:notation	RIV/60076658:12520/11:43879713!RIV12-MZE-12520___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(IAA608030801), P(LC06073), P(ME10015), P(QH82119), S, Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Alavi, Sayyed Mohammad Hadi Linhart, Otomar Gela, David Rodina, Marek Boryshpolets, Sergey Dzyuba, Boris
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	192327
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/11:43879713
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biology; methanol; motility; sturgeon; Sperm cryopreservation (en)
http://linked.open.../riv/klicoveSlovo	Sperm cryopreservation biology methanol motility sturgeon
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[411BE4E6EA81]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Ichthyology
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Biodiversity Research Center Application of Paddlefish Sex-reversal and Proteomics in Its Cryopreserved Sperm for Caviar Production Research of sperm and embryos cryopreservation
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	27
http://linked.open...iv/tvurceVysledku	Alavi, Sayyed Mohammad Hadi Boryshpolets, Sergey Dzyuba, Boris Gela, David Linhart, Otomar Rodina, Marek
http://linked.open...ain/vavai/riv/wos	000295013300002
http://linked.open...n/vavai/riv/zamer	Biologické, enviromentální a chovatelské aspekty v rybářství
issn	0175-8659
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1439-0426.2011.01866.x
http://localhost/t...ganizacniJednotka	12520

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