About: In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sexually mature pigs are known to possess high concentrations of testicular steroids, which have been shown to change the activities of cytochrome P450 in vitro. The aim of the present study was to evaluate the regulation of CYP1A and CYP2E1 activity by the steroids dihydrotestosterone (DHT), 3beta-androstenol, 17beta-estradiol and 17alfa-estradiol. Catalytic activities of 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) were used as markers of CYP1A activities, while p-nitrophenol hydroxylace (PNPH) was used as a marker of CYP2E1 activities. Of the tested steroids, only 17beta-estradiol and 17alfa-estradiol inhibited EROD and MROD activities. This inhibition was observed when a steroid concentration of 100 mýM was used, while lower concentration showed no inhibitory effect. PNPH activities were inhibited only by 100 mýM of 17beta-estradiol. The significance of those results in vivo is unknown because inhibition was only found when concentrations of oestrogens higher than physiological levels were used. Sexually mature pigs are known to possess high concentrations of testicular steroids, which have been shown to change the activities of cytochrome P450 in vitro. The aim of the present study was to evaluate the regulation of CYP1A and CYP2E1 activity by the steroids dihydrotestosterone (DHT), 3beta-androstenol, 17beta-estradiol and 17alfa-estradiol. Catalytic activities of 7-ethoxyresorufin O-deethylase (EROD) and 7-methoxyresorufin O-demethylase (MROD) were used as markers of CYP1A activities, while p-nitrophenol hydroxylace (PNPH) was used as a marker of CYP2E1 activities. Of the tested steroids, only 17beta-estradiol and 17alfa-estradiol inhibited EROD and MROD activities. This inhibition was observed when a steroid concentration of 100 mýM was used, while lower concentration showed no inhibitory effect. PNPH activities were inhibited only by 100 mýM of 17beta-estradiol. The significance of those results in vivo is unknown because inhibition was only found when concentrations of oestrogens higher than physiological levels were used. (en)
Title	In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol (en)
skos:prefLabel	In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol In vitro inhibition of porcine cytochrome P450 by 17beta -estradiol and 17alfa-estradiol (en)
skos:notation	RIV/60076658:12520/11:43872218!RIV12-MSM-12520___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), S
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Žlábek, Vladimír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	204043
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/11:43872218
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	steroid hormones; cytochrome P450; microsomes; liver; pig (en)
http://linked.open.../riv/klicoveSlovo	microsomes steroid hormones cytochrome P450 liver pig
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[B9998CCA4C3D]
http://linked.open...i/riv/nazevZdroje	Interdisciplinary Toxicology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Žlábek, Vladimír Ekstrand, Bo Herbin, Isabelle Rassmussen, Martin K. Zamaratskaia, Galia
issn	1337-6853
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/v10102-011-0014-x
http://localhost/t...ganizacniJednotka	12520

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