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rdf:type
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Description
| - Sturgeon and paddlefish (Acipenseriformes) are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish. Protein profiles of spermatozoa were determined by 2-DE electrophoresis and by MALDI-TOF/MS. Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. The glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.
- Sturgeon and paddlefish (Acipenseriformes) are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish. Protein profiles of spermatozoa were determined by 2-DE electrophoresis and by MALDI-TOF/MS. Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. The glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level. (en)
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Title
| - Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)
- Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) (en)
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skos:prefLabel
| - Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)
- Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces) (en)
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skos:notation
| - RIV/60076658:12520/10:00011455!RIV11-MSM-12520___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(ED2.1.00/01.0024), P(IAA608030801), P(LC06073), P(LC07017), P(ME10015), P(QH82119), P(QH92308), S, Z(AV0Z50200510), Z(MSM6007665809)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12520/10:00011455
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Endangered species; Sperm; Comparative proteomics; MALDI-TOF/MS (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Comparative Biochemistry and Physiology D-Genomics & Proteomics
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Hulák, Martin
- Li, Ping
- Li, Zhihua
- Linhart, Otomar
- Rodina, Marek
- Šulc, M.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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