About: Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity

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About: Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We attempted to select a fraction of common carp, Cyprinus carpio spermatozoa that best survived a conventional freeze/thaw procedure, by centrifugation of frozen/thawed sperm through a Percoll gradient (45% and 90%). The proportion of motile spermatozoa (65.81 +/- 5.19%), their velocity (77.58 +/- 31.07 mu m/sec), and membrane integrity (83.66 +/- 4.38% intact) were significantly higher in separated sperm than in whole samples (motility 23.36 +/- 2.98%, velocity 55.55 +/- 19.03 mu m/sec, and membrane integrity 57.92 +/- 4.65%). Our results demonstrated that Percoll gradient centrifugation shows promise as a technique for selecting high quality cryopreserved fish spermatozoa, which could be useful for cryobiological research. Further studies are needed to evaluate the potentially higher fertilizing ability of the separated spermatozoa. We attempted to select a fraction of common carp, Cyprinus carpio spermatozoa that best survived a conventional freeze/thaw procedure, by centrifugation of frozen/thawed sperm through a Percoll gradient (45% and 90%). The proportion of motile spermatozoa (65.81 +/- 5.19%), their velocity (77.58 +/- 31.07 mu m/sec), and membrane integrity (83.66 +/- 4.38% intact) were significantly higher in separated sperm than in whole samples (motility 23.36 +/- 2.98%, velocity 55.55 +/- 19.03 mu m/sec, and membrane integrity 57.92 +/- 4.65%). Our results demonstrated that Percoll gradient centrifugation shows promise as a technique for selecting high quality cryopreserved fish spermatozoa, which could be useful for cryobiological research. Further studies are needed to evaluate the potentially higher fertilizing ability of the separated spermatozoa. (en)
Title	Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity (en)
skos:prefLabel	Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity (en)
skos:notation	RIV/60076658:12520/10:00011443!RIV11-MSM-12520___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(IAA608030801), P(LC06073), P(QH82118), P(QH82119), P(QH92308), S, Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Linhart, Otomar Hulák, Martin Rodina, Marek Boryshpolets, Sergey Dzyuba, Boris Li, Ping Li, Zhihua
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	278461
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/10:00011443
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cyprinus carpio; cryopreservation; Percoll gradient separation; sperm; fish (en)
http://linked.open.../riv/klicoveSlovo	Cyprinus carpio cryopreservation fish sperm Percoll gradient separation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[582700DB4582]
http://linked.open...i/riv/nazevZdroje	Theriogenology
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Biodiversity Research Center Research of sperm and embryos cryopreservation Maintenance of biodiversity in cultured breeds of common carp. Genetic diversity of sterlet (Acipenser ruthenus): implication for in situ conservation and protection of biodiversity
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	74
http://linked.open...iv/tvurceVysledku	Boryshpolets, Sergey Dzyuba, Boris Hulák, Martin Li, Ping Li, Zhihua Linhart, Otomar Rodina, Marek
http://linked.open...ain/vavai/riv/wos	000283265000006
http://linked.open...n/vavai/riv/zamer	Biologické, enviromentální a chovatelské aspekty v rybářství
issn	0093-691X
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	12520

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