About: Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques

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About: Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cryopreservation causes osmotic changes and oxidative damage that have sublethal and lethal effects on spermatozoa. We examined these osmotic and oxidative effects on common carp spermatozoa motility; membrane integrity; levels of thiobarbituric-acid-reactive substance (TBARS) and carbonyl groups (CP); and the activity of superoxide dismutase (SOD), glutathione reductase, and glutathione peroxidase (GPx). Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol based extenders, followed by equilibration, freezing, and thawing. Equilibration in DMSO extender resulted in a significant reduction of spermatozoa motility, but motility was induced in those spermatozoa following dilution with saline buffer, which usually inhibits undiluted spermatozoa motility. Spermatozoa velocity and membrane integrity decreased with both extenders following freezing and thawing. No significant difference in levels of TBARS or CP, or in SOD activity, was seen in samples equilibrated with either extender. The free Cryopreservation causes osmotic changes and oxidative damage that have sublethal and lethal effects on spermatozoa. We examined these osmotic and oxidative effects on common carp spermatozoa motility; membrane integrity; levels of thiobarbituric-acid-reactive substance (TBARS) and carbonyl groups (CP); and the activity of superoxide dismutase (SOD), glutathione reductase, and glutathione peroxidase (GPx). Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol based extenders, followed by equilibration, freezing, and thawing. Equilibration in DMSO extender resulted in a significant reduction of spermatozoa motility, but motility was induced in those spermatozoa following dilution with saline buffer, which usually inhibits undiluted spermatozoa motility. Spermatozoa velocity and membrane integrity decreased with both extenders following freezing and thawing. No significant difference in levels of TBARS or CP, or in SOD activity, was seen in samples equilibrated with either extender. The free (en)
Title	Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques (en)
skos:prefLabel	Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques (en)
skos:notation	RIV/60076658:12520/10:00011442!RIV11-MSM-12520___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(IAA608030801), P(LC06073), P(ME10015), P(QH82118), P(QH82119), P(QH92308), S, Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Linhart, Otomar Hulák, Martin Rodina, Marek Dzyuba, Boris Li, Ping Li, Zhihua
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	257779
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/10:00011442
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Antioxidant; cryopreservation; cryoprotectant; osmotic stress; sperm; spermatozoa motility; stress (en)
http://linked.open.../riv/klicoveSlovo	cryopreservation sperm stress Antioxidant osmotic stress spermatozoa motility cryoprotectant
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[71F7F1A73DCD]
http://linked.open...i/riv/nazevZdroje	Biology of Reproduction
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Biodiversity Research Center Application of Paddlefish Sex-reversal and Proteomics in Its Cryopreserved Sperm for Caviar Production Research of sperm and embryos cryopreservation Maintenance of biodiversity in cultured breeds of common carp. Genetic diversity of sterlet (Acipenser ruthenus): implication for in situ conservation and protection of biodiversity
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	83
http://linked.open...iv/tvurceVysledku	Dzyuba, Boris Hulák, Martin Li, Ping Li, Zhihua Linhart, Otomar Rodina, Marek
http://linked.open...ain/vavai/riv/wos	000283398900021
http://linked.open...n/vavai/riv/zamer	Biologické, enviromentální a chovatelské aspekty v rybářství
issn	0006-3363
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	12520

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