About: Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 +/- 5.63 to 105.78 +/- 5.27 mu ms-1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 +/- 0.16 mg ml-1), spermatozoa concentration (0.28 +/- 0.27 x 109 spz ml-1), seminal plasma osmolality (51.33 +/- 4.91 mOsmol kg-1), the pH (8.49 +/- 0.01) and Na+ (18.97 +/- 3.65 mM), K+ (2.83 +/- 1.36 mM), Ca2+ (0.19 +/- 0.06 mM), Mg2+ (0.49 +/- 0.23 mM) and Cl) (6.33 +/- 0.58 mM) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and isoelectric points ranging from 5 to 9.5 were found. The observed physiological and bio Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 +/- 5.63 to 105.78 +/- 5.27 mu ms-1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 +/- 0.16 mg ml-1), spermatozoa concentration (0.28 +/- 0.27 x 109 spz ml-1), seminal plasma osmolality (51.33 +/- 4.91 mOsmol kg-1), the pH (8.49 +/- 0.01) and Na+ (18.97 +/- 3.65 mM), K+ (2.83 +/- 1.36 mM), Ca2+ (0.19 +/- 0.06 mM), Mg2+ (0.49 +/- 0.23 mM) and Cl) (6.33 +/- 0.58 mM) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and isoelectric points ranging from 5 to 9.5 were found. The observed physiological and bio (en)
Title	Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso (en)
skos:prefLabel	Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso (en)
skos:notation	RIV/60076658:12520/10:00011432!RIV11-GA0-12520___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED2.1.00/01.0024), P(GA523/08/0824), P(IAA608030801), P(LC06073), P(ME10015), P(QH82119), P(QH92308), S, Z(MSM6007665809)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Linhart, Otomar Hulák, Martin Gela, David Rodina, Marek Li, Ping Li, Zhihua
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Fakulta rybářství a ochrany vod
http://linked.open...dnocenehoVysledku	278925
http://linked.open...ai/riv/idVysledku	RIV/60076658:12520/10:00011432
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Sturgeons; spermatozoa concentrations; protein profile; cryopreservation (en)
http://linked.open.../riv/klicoveSlovo	cryopreservation protein profile Sturgeons spermatozoa concentrations
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[DDC5B2BD6450]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Ichthyology
http://linked.open...in/vavai/riv/obor	GL
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Relationships of ploidy level, genome and cell size in model polyploid fish with cytological and physiological impacts on conservation and culture. Diversity of Bioenergetics Pathways, Membrane Functions, Signaling Mechanisms and Proteomics of Cryopreserved Sperm of Evolutionary Different Fish Species Biodiversity Research Center Application of Paddlefish Sex-reversal and Proteomics in Its Cryopreserved Sperm for Caviar Production Research of sperm and embryos cryopreservation Genetic diversity of sterlet (Acipenser ruthenus): implication for in situ conservation and protection of biodiversity
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Gela, David Hulák, Martin Li, Ping Li, Zhihua Linhart, Otomar Rodina, Marek
http://linked.open...ain/vavai/riv/wos	000281894400023
http://linked.open...n/vavai/riv/zamer	Biologické, enviromentální a chovatelské aspekty v rybářství
issn	0175-8659
number of pages	3 (xsd:int)
http://localhost/t...ganizacniJednotka	12520

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