About: Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg(6)Pro(9)NEt sGnRHa     Goto   Sponge   NotDistinct   Permalink

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Description
  • Effects of physiological saline (PS)-dissolved or Freund's incomplete adjuvant (FIA)-emulsified sGnRHa on the induction and advancement of ovulation in brook char were assessed. Two-year-old females were randomly divided into 5 groups. Groups A and B received intraperitoneal injection of FIA-emulsified sGnRHa (sGnRHa-FIA) at dosages of 50 and 25 mu g kg(-1) body weight (BW), respectively. Females in group C were treated with a double injection (DI) of PS-dissolved sGnRHa (sGnRHa-PS) at 25 mu g kg(-1) BW spaced 3 days apart. Fish in group D received a 25 mu g kg(-1) BW single injection (SI) of sGnRHa-PS. Group E was established as a control group. After stripping, ovarian plasma pH level was measured, and an egg sample was taken from each female to record egg weight and diameter and survival to the eyed stage. Females in the GnRHa-treated groups ovulated significantly earlier than did females in the control group (P { 0.01). No significant differences were found among GnRHa-treated groups in ovulation dynamics and mean time to ovulation. Ovarian fluid pH was significantly higher in groups A, B, and C compared to control group E (P { 0.05). Significantly lower egg weight was found only in group B (P { 0.01), although all advanced groups tended to have lower egg weight than the control group. Egg diameters paralleled egg weight. Survival to the eyed stage was significantly higher in GnRHa-advanced groups compared to the control. A negative relationship was found between egg weight end eyed eggs percent (R (2) = 0.26). No pre-spawning or post-spawning mortality was observed during a 6-month period. Neither sGnRHa-PS nor sGnRHa-FIA are associated with negative influences on the health of females. The sGnRHa-FIA injections proved to exhibit the same efficacy as the DI protocol with sGnRHa-PS. Although no statistical differences were found in ovulation dynamics, we do not recommend the use of SI with sGnRHa-PS in brook char.
  • Effects of physiological saline (PS)-dissolved or Freund's incomplete adjuvant (FIA)-emulsified sGnRHa on the induction and advancement of ovulation in brook char were assessed. Two-year-old females were randomly divided into 5 groups. Groups A and B received intraperitoneal injection of FIA-emulsified sGnRHa (sGnRHa-FIA) at dosages of 50 and 25 mu g kg(-1) body weight (BW), respectively. Females in group C were treated with a double injection (DI) of PS-dissolved sGnRHa (sGnRHa-PS) at 25 mu g kg(-1) BW spaced 3 days apart. Fish in group D received a 25 mu g kg(-1) BW single injection (SI) of sGnRHa-PS. Group E was established as a control group. After stripping, ovarian plasma pH level was measured, and an egg sample was taken from each female to record egg weight and diameter and survival to the eyed stage. Females in the GnRHa-treated groups ovulated significantly earlier than did females in the control group (P { 0.01). No significant differences were found among GnRHa-treated groups in ovulation dynamics and mean time to ovulation. Ovarian fluid pH was significantly higher in groups A, B, and C compared to control group E (P { 0.05). Significantly lower egg weight was found only in group B (P { 0.01), although all advanced groups tended to have lower egg weight than the control group. Egg diameters paralleled egg weight. Survival to the eyed stage was significantly higher in GnRHa-advanced groups compared to the control. A negative relationship was found between egg weight end eyed eggs percent (R (2) = 0.26). No pre-spawning or post-spawning mortality was observed during a 6-month period. Neither sGnRHa-PS nor sGnRHa-FIA are associated with negative influences on the health of females. The sGnRHa-FIA injections proved to exhibit the same efficacy as the DI protocol with sGnRHa-PS. Although no statistical differences were found in ovulation dynamics, we do not recommend the use of SI with sGnRHa-PS in brook char. (en)
Title
  • Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg(6)Pro(9)NEt sGnRHa
  • Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg(6)Pro(9)NEt sGnRHa (en)
skos:prefLabel
  • Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg(6)Pro(9)NEt sGnRHa
  • Synchronization of ovulation in brook char (Salvelinus fontinalis, Mitchill 1814) using emulsified d-Arg(6)Pro(9)NEt sGnRHa (en)
skos:notation
  • RIV/60076658:12310/13:43885246!RIV14-MZE-12310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/01.0024), P(ME10126), P(QH71305), P(QH91310), S
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 109374
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12310/13:43885246
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • sGnRHa; d-Arg6Pro9NEt; emulsified; using; 1814; Mitchill; fontinalis; Salvelinus; char; brook; ovulation; Synchronization (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [975C6859EB9B]
http://linked.open...i/riv/nazevZdroje
  • Aquaculture International
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 21
http://linked.open...iv/tvurceVysledku
  • Kouřil, Jan
  • Policar, Tomáš
  • Švinger, Viktor William
  • Poláková, Simona
  • Steinbach, Christoph Antonius
  • Jankových, Antonín
http://linked.open...ain/vavai/riv/wos
  • 000321963200006
issn
  • 0967-6120
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10499-012-9578-5
http://localhost/t...ganizacniJednotka
  • 12310
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