About: Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei     Goto   Sponge   NotDistinct   Permalink

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Description
  • We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential.
  • We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential. (en)
Title
  • Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei
  • Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei (en)
skos:prefLabel
  • Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei
  • Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei (en)
skos:notation
  • RIV/60076658:12310/10:00011920!RIV11-MSM-12310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(LC07032), S, Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 271605
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12310/10:00011920
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • gene-function; interference; expression; subunits; library; DNA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [233CF4D8126E]
http://linked.open...i/riv/nazevZdroje
  • Parasitology Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 106
http://linked.open...iv/tvurceVysledku
  • Lukeš, Julius
  • Verner, Zdeněk
  • Paris, Zdeněk
http://linked.open...ain/vavai/riv/wos
  • 000276234300030
http://linked.open...n/vavai/riv/zamer
issn
  • 0932-0113
number of pages
http://localhost/t...ganizacniJednotka
  • 12310
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