About: Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification

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About: Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	RT-PCR specifická pro tick-borne encephalitis virus (cs) Among diseases transmitted by ticks in the Czech Republic. tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks based on the detection of TBEV RNA using an RT-PCR technique without RNA purification. The method is very sensitive with the detection limit of about 14 fg TBEV RNA in total RNA obtained from brain suspension from suckling mice infected with TBEV per reaction. The primers were derived from the 5'-terminal non-coding region, a highly conserved part of the virus. The method was successfully applied to field-collected ticks in detecting TBEV RNA. This method can be used in studies of several aspects of TBEV: epidemiology. screening of natural foci, circulation and detection of virus genome sequences in clinical materials. Among diseases transmitted by ticks in the Czech Republic. tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks based on the detection of TBEV RNA using an RT-PCR technique without RNA purification. The method is very sensitive with the detection limit of about 14 fg TBEV RNA in total RNA obtained from brain suspension from suckling mice infected with TBEV per reaction. The primers were derived from the 5'-terminal non-coding region, a highly conserved part of the virus. The method was successfully applied to field-collected ticks in detecting TBEV RNA. This method can be used in studies of several aspects of TBEV: epidemiology. screening of natural foci, circulation and detection of virus genome sequences in clinical materials. (en)
Title	RT-PCR specifická pro tick-borne encephalitis virus (cs) Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification (en)
skos:prefLabel	RT-PCR specifická pro tick-borne encephalitis virus (cs) Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification Tick-borne encephalitis virus-specific RT-PCR - A rapid test for detection of the pathogen without viral RNA purification (en)
skos:notation	RIV/60076658:12310/04:00008659!RIV09-MSM-12310___
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(GA524/03/1326), V, Z(AV0Z6022909)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Grubhoffer, Libor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	590339
http://linked.open...ai/riv/idVysledku	RIV/60076658:12310/04:00008659
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NATURAL FOCI; SEQUENCE; INFECTION; SIBERIA; TBEV; ASIA (en)
http://linked.open.../riv/klicoveSlovo	TBEV SEQUENCE INFECTION NATURAL FOCI ASIA SIBERIA
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[F06F4C5AD08B]
http://linked.open...i/riv/nazevZdroje	Acta Virologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular typing and genetic characterization of Lyme borreliosis spirochetes.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Grubhoffer, Libor Rudenko, N. Golovchenko, M. Cihlářová, V.
http://linked.open...ain/vavai/riv/wos	000226306300005
http://linked.open...n/vavai/riv/zamer	Parazitické a symbiotické organismy živočichů a člověka: jejich vzájemné vztahy na úrovni populací, organismů, buněk a molekul
issn	0001-723X
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	12310

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