About: Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria

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About: Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens. We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007- 2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57%) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens. (en)
Title	Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria (en)
skos:prefLabel	Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria (en)
skos:notation	RIV/60076658:12220/15:43887835!RIV15-GA0-12220___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.3.30.0049), P(GD521/08/H042)
http://linked.open...iv/cisloPeriodika	141
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Bohatá, Andrea Mráz, Ivan Beran, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	287
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/15:43887835
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	atpD,Oligonucleotides, Xanthomonas euvesicatoria, Lycopersicon esculentum, Capsicum annuum (en)
http://linked.open.../riv/klicoveSlovo	Capsicum annuum atpD Lycopersicon esculentum Xanthomonas euvesicatoria Oligonucleotides
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[47601AA08D9F]
http://linked.open...i/riv/nazevZdroje	European Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular biology, ecology and taxonomy Development of postdoc positions on USB
http://linked.open...UplatneniVysledku	2015
http://linked.open...v/svazekPeriodika	2015
http://linked.open...iv/tvurceVysledku	Beran, Pavel Kokošková, Blanka Mráz, Ivan Bohatá, Andrea
issn	0929-1873
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10658-014-0554-y
http://localhost/t...ganizacniJednotka	12220

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