About: The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi     Goto   Sponge   NotDistinct   Permalink

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  • The contribution deals with the assessments of antifungal potential of thermostable proteins from three potato cultivars (Solanum tuberosum L.) David, Marabel and Westamyl differ in the market and maturity and comparison of two potato fruit juice alternatives differing in processing temperature. Combination of pH adjustment and high temperature (80 °C) or pH adjustment only was used for protein isolation. Isolated proteins were detected by peptide SDS-PAGE in range from 6 to 14 kDa and in ranges 14 and 17 kDa in the sample of the thermally untreated potato fruit juice. Three different concentrations of protein samples were applied to three different varieties of phytopathogenic fungi Alternaria solani, Fusarium solani and Rhizoctonia solani. The inhibition of mycelial growth was evaluated after 24 hours of growth at 16 °C on digital photos by special software using the method of measuring the inhibition area. The greatest inhibition area (108,9 mm2) was achieved on F. solani cultivar David at concentration 10 mg/ml (inhibition area of Marabel 86,1 mm2 and Westamyl 103,3 mm2). No antifungal affect was achieved at cultivars Marabel and Westamyl at concentration 1 mg/ml at the phytopathogenic fungi A. solani (inhibition area of David was 5.9 mm2). The phytopathogenic fungi F. solani and R. solani were more sensitive to thermostable proteins in general and inhibition effect of thermally untreated substance was not registered.
  • The contribution deals with the assessments of antifungal potential of thermostable proteins from three potato cultivars (Solanum tuberosum L.) David, Marabel and Westamyl differ in the market and maturity and comparison of two potato fruit juice alternatives differing in processing temperature. Combination of pH adjustment and high temperature (80 °C) or pH adjustment only was used for protein isolation. Isolated proteins were detected by peptide SDS-PAGE in range from 6 to 14 kDa and in ranges 14 and 17 kDa in the sample of the thermally untreated potato fruit juice. Three different concentrations of protein samples were applied to three different varieties of phytopathogenic fungi Alternaria solani, Fusarium solani and Rhizoctonia solani. The inhibition of mycelial growth was evaluated after 24 hours of growth at 16 °C on digital photos by special software using the method of measuring the inhibition area. The greatest inhibition area (108,9 mm2) was achieved on F. solani cultivar David at concentration 10 mg/ml (inhibition area of Marabel 86,1 mm2 and Westamyl 103,3 mm2). No antifungal affect was achieved at cultivars Marabel and Westamyl at concentration 1 mg/ml at the phytopathogenic fungi A. solani (inhibition area of David was 5.9 mm2). The phytopathogenic fungi F. solani and R. solani were more sensitive to thermostable proteins in general and inhibition effect of thermally untreated substance was not registered. (en)
Title
  • The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi
  • The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi (en)
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  • The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi
  • The assessment of thermostable proteins gained from different potato tuber cultivars (Solanum tuberosum L.) and their influence on growth inhibition of selected phytopathogenic fungi (en)
skos:notation
  • RIV/60076658:12220/13:43886342!RIV14-GA0-12220___
http://linked.open...avai/riv/aktivita
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  • P(GA522/09/1693), S
http://linked.open...vai/riv/dodaniDat
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  • 62320
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  • RIV/60076658:12220/13:43886342
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  • cultivar variability; thermostable proteins; antifungal activity; Solanum tuberosum L.; potato fruit juice (en)
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  • [24E0271C2D76]
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  • Praha
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  • Potato Research Institute Havlíčkův Brod
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  • Proceedings of the 2nd International Symposium on Agronomy and Physiology of Potato
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  • Bárta, Jan
  • Bártová, Veronika
  • Kamenová, Andrea
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http://linked.open.../riv/zahajeniAkce
number of pages
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  • Tisk Hermann spol.
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  • 978-80-86940-52-6
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  • 12220
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