About: Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography     Goto   Sponge   NotDistinct   Permalink

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  • The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin.
  • The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin. (en)
Title
  • Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
  • Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography (en)
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  • Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography
  • Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography (en)
skos:notation
  • RIV/60076658:12220/10:00012481!RIV11-MSM-12220___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 253798
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  • RIV/60076658:12220/10:00012481
http://linked.open...riv/jazykVysledku
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  • buckwheat; MEKC; quercetin glycosides (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [253166520751]
http://linked.open...i/riv/nazevZdroje
  • Journal of Separation Science
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 33
http://linked.open...iv/tvurceVysledku
  • Dadáková, Eva
  • Kalinová, Jana
http://linked.open...ain/vavai/riv/wos
  • 000278829700014
http://linked.open...n/vavai/riv/zamer
issn
  • 1615-9306
number of pages
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  • 12220
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