About: Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A rapid ultra-performance liquid chromatographic (UPLC) method for the determination of biogenic amines (putrescine, cadaverine, spermidine, spermine, phenylethylamine, histamine, tyramine and tryptamine) in selected food samples is described. The 8 biogenic amines, which are the most important to be determined in food samples, were derivatized with dansyl chloride prior to UPLC separation. The dansylated amines were separated on an Agilent Zorbax Eclipse XDB ? C18 column (50 x 4,6 mm I.D., 1.8 m) using gradient elution with a binary system of acetonitrile-water, a flow-rate of 1.0 ml/min and UV detection at 225 nm. The average repeatability of the method ranged between 1.02 ? 2.14 %. Limits of detection (LODs) ranged between 0.032 ? 0.098 g/l; calibration curves showed very good linearity (r = 0.9994 ? 1.0000). The method was applied to the analysis of amines in pork, beef, chicken and fish meat, cheese and edible mushrooms. A rapid ultra-performance liquid chromatographic (UPLC) method for the determination of biogenic amines (putrescine, cadaverine, spermidine, spermine, phenylethylamine, histamine, tyramine and tryptamine) in selected food samples is described. The 8 biogenic amines, which are the most important to be determined in food samples, were derivatized with dansyl chloride prior to UPLC separation. The dansylated amines were separated on an Agilent Zorbax Eclipse XDB ? C18 column (50 x 4,6 mm I.D., 1.8 m) using gradient elution with a binary system of acetonitrile-water, a flow-rate of 1.0 ml/min and UV detection at 225 nm. The average repeatability of the method ranged between 1.02 ? 2.14 %. Limits of detection (LODs) ranged between 0.032 ? 0.098 g/l; calibration curves showed very good linearity (r = 0.9994 ? 1.0000). The method was applied to the analysis of amines in pork, beef, chicken and fish meat, cheese and edible mushrooms. (en)
Title	Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC) Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC) (en)
skos:prefLabel	Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC) Determination of biogenic amines in foods using ultra-performance liquid chromatography (UPLC) (en)
skos:notation	RIV/60076658:12220/09:00010865!RIV10-MSM-12220___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Dadáková, Eva Pelikánová, Tamara Křížek, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	309881
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/09:00010865
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	UPLC; HPLC; biogenic amines; determination; foods; putrescine; histamine (en)
http://linked.open.../riv/klicoveSlovo	putrescine UPLC foods HPLC biogenic amines determination histamine
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[D2430FCA86DA]
http://linked.open...i/riv/nazevZdroje	Food Chemistry
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	116
http://linked.open...iv/tvurceVysledku	Dadáková, Eva Křížek, Martin Pelikánová, Tamara
http://linked.open...n/vavai/riv/zamer	Sustainable methods of agricultural management in montane and submontane areas with respect to harmonization of the production and nonproduction spheres
issn	0308-8146
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	12220

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