About: Identification of white clover (Trifolium repens L.) cultivars using molecular markers.     Goto   Sponge   NotDistinct   Permalink

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Description
  • V letech 2002-2003 byly studovány různé metody molekulární analýzy pro identifikaci vybraných populací jetele plazivého (Trifolium repens L.). Pro studium byly použity RAPD, SSR (mikrosatelity), rDNA a PCR-RFLP markery. RAPD analýzou byla detekována vysoká genetická variabilita mezi odrůdami, ale i uvnitř odrůdy. RAPD markery se ukázaly jako nejméně vhodný markerovací systém pro determinaci odrůd jetele plazivého. Nízká variabilita rDNA a PCR-RFLP markerů neumožnila rozlišení odrůd. Pomocí SSR a rDNA markerů nebyla zjištěna mezi rostlinami jedné odrůdy žádná genetická variabilita. Mikrosatelitovou analýzou byly amplifikovány rozdílné velikosti PCR fragmentů vhodné pro determinaci odrůd. (cs)
  • The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of pa?erns within one cultivar. The different sizes of PCR fragments were obtained a?er amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars.
  • The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of pa?erns within one cultivar. The different sizes of PCR fragments were obtained a?er amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars. (en)
Title
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers.
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers. (en)
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers. (cs)
skos:prefLabel
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers.
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers. (en)
  • Identification of white clover (Trifolium repens L.) cultivars using molecular markers. (cs)
skos:notation
  • RIV/60076658:12220/04:00005257!RIV06-MZE-12220___
http://linked.open.../vavai/riv/strany
  • 95-100
http://linked.open...avai/riv/aktivita
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  • P(QC1074), Z(MSM 122200002), Z(MSM 122200004)
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  • 3
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  • 567192
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  • RIV/60076658:12220/04:00005257
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  • White clover; Trifolium repens L.; cultivars; RAPD, SSR, rDNA, PCR-RFLP markers; differences; determination; suitable markers (en)
http://linked.open.../riv/klicoveSlovo
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  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [C58D01783DA5]
http://linked.open...i/riv/nazevZdroje
  • Plant, Soil and Environment
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Čurn, Vladislav
  • Dolanská, Lenka
http://linked.open...n/vavai/riv/zamer
issn
  • 1214-1178
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  • 12220
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