About: Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.)     Goto   Sponge   NotDistinct   Permalink

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  • Esterase isozymes, proteins extracted in 2% glutathione buffer (separated in non denaturing PAGE system) and seed proteins separated in SDS page were used as biochemical markers for evaluation of polymorphysm level in three spelt wheat varieties, three breeders spelt lines (orig. from hybrids. between spelt and common wheat) and reference common wheat variety. Electrophoresis phenotypes and zymograms were evaluated by means of digital image analysis and Nei + Li coufficient if similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of three marker systems showed differences between common wheat variety Brea and spelt varieties and spelt breeders´ lines. Also significant differences between old spelt varieties (Hercule, Altgold and Rouquin) and new spelt breeders´ lines were found.
  • Esterase isozymes, proteins extracted in 2% glutathione buffer (separated in non denaturing PAGE system) and seed proteins separated in SDS page were used as biochemical markers for evaluation of polymorphysm level in three spelt wheat varieties, three breeders spelt lines (orig. from hybrids. between spelt and common wheat) and reference common wheat variety. Electrophoresis phenotypes and zymograms were evaluated by means of digital image analysis and Nei + Li coufficient if similarity was used to evaluate the relation of analysed genotypes. Entire evaluation of three marker systems showed differences between common wheat variety Brea and spelt varieties and spelt breeders´ lines. Also significant differences between old spelt varieties (Hercule, Altgold and Rouquin) and new spelt breeders´ lines were found. (en)
Title
  • Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.)
  • Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.) (en)
skos:prefLabel
  • Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.)
  • Methodological approach to evaluation of biochemical markers in spelt wheat (Triticum spelta L.) (en)
skos:notation
  • RIV/60076658:12220/01:00002839!RIV/2002/GA0/122202/N
http://linked.open.../vavai/riv/strany
  • 171-182
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA303/01/1380), Z(MSM 122200002)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
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http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 686622
http://linked.open...ai/riv/idVysledku
  • RIV/60076658:12220/01:00002839
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • pšenice špalda; biochemické markery; zásobní proteiny; isoenzymy; elektroforéza (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [DA2C0D233F81]
http://linked.open...i/riv/nazevZdroje
  • Scientia agriculturae bohemica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 32
http://linked.open...iv/tvurceVysledku
  • Moudrý, Jan
  • Dvořáček, Václav
  • Čurn, Vladislav
http://linked.open...n/vavai/riv/zamer
issn
  • 0582-2343
number of pages
http://localhost/t...ganizacniJednotka
  • 12220
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