About: Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells

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About: Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Objectives: Many pathways seem to be involved in the regulation of the intra-S-phase checkpoint after exposure to ionizing radiation, but the role of p53 has proven to be rather elusive. Here we have a closer look at the progression of irradiated cells through S-phase in dependence of their p53 status. Materials and methods: Three pairs of cell lines were used, each consisting of one p53 functional and one p53 non-functional line. Cells were labelled with BrdU immediately after irradiation, they were then incubated in label-free medium, and at different times afterwards their position within the S-phase was determined flow cytometrically. Results: While in the p53 deficient cells progression through S-phase was slowed significantly over at least a few hours, it was halted for just about an hour in the p53 proficient cells and then proceeded without further delay or even at a slightly accelerated pace. Conclusions: It is clear from the experiments presented here that p53 does play a role for the progress of cells through the S-phase after X-ray exposure, but the exact mechanisms by which replicon initiation and elongation is controlled in irradiated cells remain to be elucidated. Objectives: Many pathways seem to be involved in the regulation of the intra-S-phase checkpoint after exposure to ionizing radiation, but the role of p53 has proven to be rather elusive. Here we have a closer look at the progression of irradiated cells through S-phase in dependence of their p53 status. Materials and methods: Three pairs of cell lines were used, each consisting of one p53 functional and one p53 non-functional line. Cells were labelled with BrdU immediately after irradiation, they were then incubated in label-free medium, and at different times afterwards their position within the S-phase was determined flow cytometrically. Results: While in the p53 deficient cells progression through S-phase was slowed significantly over at least a few hours, it was halted for just about an hour in the p53 proficient cells and then proceeded without further delay or even at a slightly accelerated pace. Conclusions: It is clear from the experiments presented here that p53 does play a role for the progress of cells through the S-phase after X-ray exposure, but the exact mechanisms by which replicon initiation and elongation is controlled in irradiated cells remain to be elucidated. (en)
Title	Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells (en)
skos:prefLabel	Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells Differential S-phase progression after irradiation of p53 functional versus non-functional tumor cells (en)
skos:notation	RIV/60076658:12110/14:43887832!RIV15-MSM-12110___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zölzer, Friedo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zdravotně sociální fakulta
http://linked.open...dnocenehoVysledku	11493
http://linked.open...ai/riv/idVysledku	RIV/60076658:12110/14:43887832
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	relative movement; flow cytometry; intra-S-phase checkpoint; X-rays (en)
http://linked.open.../riv/klicoveSlovo	intra-S-phase checkpoint relative movement flow cytometry X-rays
http://linked.open...odStatuVydavatele	SI - Slovinská republika
http://linked.open...ontrolniKodProRIV	[3CD431D131F5]
http://linked.open...i/riv/nazevZdroje	Radiology and Oncology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Zölzer, Friedo Streffer, Christian Musseldt, Tamara
http://linked.open...ain/vavai/riv/wos	000344946200004
issn	1318-2099
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/raon-2014-0032
http://localhost/t...ganizacniJednotka	12110

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