About: Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell

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About: Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	PURPOSE Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test this hypothesis. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay and two-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C. RESULTS The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediately before or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected. CONCLUSIONS The protective effect of hypothermia observed at the level of cytogenetic damage was not due to a modulation of cell cycle progression. A possible alternative mechanism and experiments to test it are discussed. PURPOSE Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test this hypothesis. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay and two-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C. RESULTS The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediately before or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected. CONCLUSIONS The protective effect of hypothermia observed at the level of cytogenetic damage was not due to a modulation of cell cycle progression. A possible alternative mechanism and experiments to test it are discussed. (en)
Title	Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell (en)
skos:prefLabel	Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell (en)
skos:notation	RIV/60076658:12110/14:43887830!RIV15-MSM-12110___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zölzer, Friedo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zdravotně sociální fakulta
http://linked.open...dnocenehoVysledku	13342
http://linked.open...ai/riv/idVysledku	RIV/60076658:12110/14:43887830
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Temperature; Chromosome-Defective; Micronuclei; Humans; DNA Damage; Cultured Cells; Cell Cycle (en)
http://linked.open.../riv/klicoveSlovo	DNA Damage Cell Cycle Chromosome-Defective Cultured Cells Temperature Micronuclei Humans
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[CEC67B0F1481]
http://linked.open...i/riv/nazevZdroje	International Journal of Radiation Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	90
http://linked.open...iv/tvurceVysledku	Zölzer, Friedo Brehwens, Karl Czub, Jan Haghdoost, Siamak Lankoff, Anna Lisowska, Halina Wegierek-Ciuk, Anna Wojcik, Andrzej
http://linked.open...ain/vavai/riv/wos	000333582100007
issn	0955-3002
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.3109/09553002.2014.887233
http://localhost/t...ganizacniJednotka	12110

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