About: Development of a dip-stick electrochemical micro-biosensor: Stability of protein layers on gold

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0167931713001020#
Description	We report on different strategies for creating a stable biomolecular layer on the surface of a gold electrode tip. The gold electrode is embedded within an epoxy casing to create a dip-stick like and easy-to-handle probe for biosensing purposes. We employ passive adsorption, passive adsorption followed by crosslinking with glutardialdehyde, and covalent linking of biomolecules via thiol-based self-assembled monolayers to produce the biomolecular layers on the gold electrode. The stability of these layers is tested by electrochemical impedance spectroscopy (EIS) in a phosphate buffer solution with an addition of a Fe3+/Fe2+ redox couple. The results of the stability studies clearly indicate that while the passive adsorption is the easiest way to produce the biomolecule layer, it does not provide for the necessary stability of the biomolecule layer with respect to further biosensing purposes. Unlike that, the stability of biomolecular layer made with the use of the other two strategies is reasonably good, however the sensing and the recognition ability of this biomolecular layer is substantially diminished. We discuss the reasons of the observed behavior and suggest possible solutions to these problems. We report on different strategies for creating a stable biomolecular layer on the surface of a gold electrode tip. The gold electrode is embedded within an epoxy casing to create a dip-stick like and easy-to-handle probe for biosensing purposes. We employ passive adsorption, passive adsorption followed by crosslinking with glutardialdehyde, and covalent linking of biomolecules via thiol-based self-assembled monolayers to produce the biomolecular layers on the gold electrode. The stability of these layers is tested by electrochemical impedance spectroscopy (EIS) in a phosphate buffer solution with an addition of a Fe3+/Fe2+ redox couple. The results of the stability studies clearly indicate that while the passive adsorption is the easiest way to produce the biomolecule layer, it does not provide for the necessary stability of the biomolecule layer with respect to further biosensing purposes. Unlike that, the stability of biomolecular layer made with the use of the other two strategies is reasonably good, however the sensing and the recognition ability of this biomolecular layer is substantially diminished. We discuss the reasons of the observed behavior and suggest possible solutions to these problems. (en)
Title	Development of a dip-stick electrochemical micro-biosensor: Stability of protein layers on gold Development of a dip-stick electrochemical micro-biosensor: Stability of protein layers on gold (en)
skos:prefLabel	Development of a dip-stick electrochemical micro-biosensor: Stability of protein layers on gold Development of a dip-stick electrochemical micro-biosensor: Stability of protein layers on gold (en)
skos:notation	RIV/49777513:23640/13:43919900!RIV14-MSM-23640___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/03.0088), S
http://linked.open...iv/cisloPeriodika	listopad
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šnita, Dalimil
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Západočeská univerzita v Plzni / Nové technologie - výzkumné centrum
http://linked.open...dnocenehoVysledku	69075
http://linked.open...ai/riv/idVysledku	RIV/49777513:23640/13:43919900
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Self-assembled monolayers; Impedance spectroscopy; Microelectrodes; Immunoassay; Microsensor (en)
http://linked.open.../riv/klicoveSlovo	Immunoassay Microsensor Self-assembled monolayers Microelectrodes Impedance spectroscopy
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[CF3C0EFCEF00]
http://linked.open...i/riv/nazevZdroje	Microelectronic Engineering
http://linked.open...in/vavai/riv/obor	CI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of the New Technologies and Materials
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	111
http://linked.open...iv/tvurceVysledku	Fišer, Jan Slouka, Zdeněk Šnita, Dalimil Přibyl, Michal Lamborová, Věra Lidner, Jiří
http://linked.open...ain/vavai/riv/wos	000322751300058
issn	0167-9317
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.mee.2013.01.059
http://localhost/t...ganizacniJednotka	23640

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